Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,003,264	C→T	E115K (GAA→AAA)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,003,264	0	C	T	100.0%	30.2 / NA	12	E115K (GAA→AAA)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

TGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAG  >  NC_000913/2003018‑2003506                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   tgtttgtGGTGGTAGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTTGTTATCATTTGCGCCAACCTGGATGTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACACGGTCAATTTCATCCAGACGGGATTTAATTTTGTc                                                                                                                                                                                                                                                 <  1:70575/250‑1 (MQ=255)                      aagaaGTTACTGGAGCACTATTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCTGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGAc                                                                                                                                                                                                                           <  1:226255/247‑1 (MQ=255)                                                                       aaacccATCAAGGCCAAGATTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACTTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCTGTAGTGGCCTGTACCGTCAGTTCACGCACACGc                                                                                                                                                                           <  1:206553/246‑1 (MQ=255)                                                                                                                                 tGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATc                                                                                                                                                                                                                               <  1:214562/140‑1 (MQ=255)                                                                                                                                        cATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCCGCACGTTTACACCGTTGAACTGGGCCTGACCAGATCCGCGGTCAATTTCATCCAGAAGGGATTTAATTTTGTCCTGGATAGAAGCCAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGAACACGCTGTAAGTTGTTCTTGATTTCGGACAgaccaccgaaggagcgctgtacg                                                                                                                           >  1:231936/1‑211 (MQ=255)                                                                                                                                                                                                  gAACTGGGTCTGACCAGATACGCGGTCAATTTCATCAAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCggtg                                                                                                                                    >  1:94606/1‑166 (MQ=255)                                                                                                                                                                                                        gggcTGACCAGATACGCGGTCAAGTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGGAGAGAGATCAGACTCAGAGTTAGTTCCGGTATTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAgc                                                                                                                                                <  1:145909/145‑1 (MQ=255)                                                                                                                                                                                                                      cgcgGTCAATTTCATCCAGACGGGATTTAATGTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGAGAGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTGACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAATCGGTTAGCAATCGCCTGACCCGCTGCGTCATcn                           <  1:160727/251‑2 (MQ=255)                                                                                                                                                                                                                            cAATTTCATCCAGCCGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACCGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTACGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTcgcg                     >  1:80154/1‑251 (MQ=255)                                                                                                                                                                                                                                     ccAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGCGTTAGTACCGGTAGTGGCCTGTACCGCCCGTACACGCACACGCTGTAAgttgt                                                                                                                                                                 >  1:165964/1‑102 (MQ=255)                                                                                                                                                                                                                                            ggATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCaaac     >  1:14474/1‑249 (MQ=255)                                                                                                                                                                                                                                                ttAATTTTGGCCTGGATAGAAGACAGATCAGACGCAGAGTTAGTGCCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGGTGTTGTTGATTTCGGACAGCGCGCCGTCGGTGGTCTGCGCAACGGAGATACCGTCTTTGGCGTTACGGGCCGCCTGAGTCAGGTCTTGTATTTGATAGGGGAAGCGGTTAGCAATCGCCTTGCCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCag  <  1:238554/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   TGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAG  >  NC_000913/2003018‑2003506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑