Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,528,294	C→T	A609V (GCT→GTT)	igaA →	inner membrane protein IgaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,528,294	0	C	T	100.0%	28.6 / NA	12	A609V (GCT→GTT)	igaA	inner membrane protein IgaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/3);  total (9/3)

GCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGC‑TGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAA‑CGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGAC‑TACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTC  >  NC_000913/3528069‑3528539                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gggtaaaTGTGTTTTTGCGCCGGGTGAGTG‑GGAAACGCCTGGAGACCCGGGTGGCATCCTCCCCCGCGCCGGTAACCGCGAATGAAC‑CGGCGGGAGGGGCACAATCACTAAACGGTCCGGCCCCCGGGGGTGCCCTGATTGTCAGCGACGAAGGCGGCGATTTGGTTGAGCAGCCGTGGCCTTGGGCATCTCTTTACGAC‑TACCCGCCGCAAGAACAGTGGAACGTTTTCCAGAAACTGGCACAAATGCtn                                                                                                                                                                                                                              <  1:53424/246‑2 (MQ=255)  cGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGC‑TGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAA‑CGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGCTTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGAC‑TACCCGCCGCAAGAACAGTGGAACGTTTTCCAGAAACTGGCACAAATg                                                                                                                                                                                                                                 >  1:162137/1‑247 (MQ=255)                                               ccctgtggccAACCTCCCCCGCGCCGTTTTTCACGC‑TGAAAACGGCGCGAGCGGCATATTCCCTAAACATCCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGAAGCTTTTTTTTTGATCATCCCGGGCCTTCGCCATCCCTTTCCGAC‑TCCCCGCCGCAAGAACAGTGGAACGTTTTCCAGAAACTGGCACAAATGCTGATGCATACTCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTc                                                                                                                                                                                 <  1:214731/242‑1 (MQ=255)                                                ccTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGACA‑CGGCGCGAGCGGCCCACACACTAACCAGTACGGACCCCGGCGGATTCCTCATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGAC‑TACCCGCCGCAAGAACAGTGGAACGTTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTc                                                                                                                                                                                 >  1:107762/1‑250 (MQ=255)                                                                                  cATGAAA‑CGGCGCGAGCGGCACAATCACTAAACAGTCCGGAACCCGGCGGATACCTGATTGTCAGCGACGAAGGCAGCAATTTTGTTGATCAGCCCTAGCCTTCGGCATCACTTTAAGAC‑TACCCGCCGCAAGAACAGTGGAACGTTTTACAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTc                                                                                                                                               >  1:232743/1‑250 (MQ=255)                                                                                  cATGAAA‑CGGCGCGAGCGGCACAATCACTAAACAGTCCGCCCCCCGGCGAATTCCTGATTGCCAGCGACGACGGCAGCGATTTTGTTATTCAGCCCTGCCCTTCGCCATCACTTTAC‑ACATACCCGCCGCAAGAACAGTGGAACGTTTTCCAGAAACTGGCACAAAGGCTGATGCAACCGCCGTTTAACGCCGAGGGATTCGCCACAAAAATTTCCACTACCGCCATTGGAACGCACCATTTTGGCCTTc                                                                                                                                               >  1:172167/1‑250 (MQ=255)                                                                                                   cGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGAC‑TACCCGCCGCAAGCACCGTGGAACGTTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTg                                                                                                                                                                             >  1:140155/1‑204 (MQ=255)                                                                                                      cacaATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGAC‑TACCCGCCGCAAGAACAGTGGAACGTTTTCCAGAAACTGGCACCAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATcc                                                                                                                                           >  1:253105/1‑235 (MQ=255)                                                                                                                                                                                    ggctttCGGCATCACTTTCCGAC‑TCCCCGCCGCAAGAACAGTGGAACGTTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAgg                                               <  1:230248/245‑1 (MQ=255)                                                                                                                                                                                             naTCACTTTACGAC‑TACCCGCCGCAAGAACAGTGGAACGTTTTCCAGAACCTGGCACAAATGCTGCTGCATACg                                                                                                                                                                                                                     >  1:32173/2‑74 (MQ=255)                                                                                                                                                                                                  cTTTACGAC‑TACCCGCCGCAAGAACAGTGGCACGTTTACCACAAACTGGAACAAATGAAGATCCATACGCCGTTTAACGACGCAGGTATCGACACAACCCTCATCAATGACGCACCTGGTAAGCAGCATATTGGCCTTCATCCGAACACGGATCGTTCCGGCCAGTGGCGCTATTTCAGGACCACATTGCTGCTCCTGACGATGCTgg                                                                          >  1:224957/1‑208 (MQ=255)                                                                                                                                                                                                                                gAACGTTTTCCAGAAACTGGCACAAATGATGAAGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTc  >  1:91281/1‑251 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGC‑TGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAA‑CGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGAC‑TACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTC  >  NC_000913/3528069‑3528539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑