Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,093,181	G→A	R82H (CGC→CAC)	yqgE →	DUF179 domain‑containing protein YqgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,093,181	0	G	A	100.0%	27.3 / NA	12	R82H (CGC→CAC)	yqgE	DUF179 domain‑containing protein YqgE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/8);  total (4/8)

CATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGAACATAATACCAATGGTGCAATGGGGATCATCGTCAACAAGCCGCTGGAAAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATTCTCGATAACGCGTGGCTAACGGCCCCGGCAGATCTGAATATTCTGTTCAAA  >  NC_000913/3092949‑3093413                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         cATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGAACATAATACCAATGGTGCAATGGGGATCATCGTCAACAAGCCGCTGGAAAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCACGGGTTTATTTTGCATAc                                                                                                                                                                                                                        <  1:135977/251‑1 (MQ=255)            tATTTCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGAACATAATACCAATGGTGCAATGGGGATCATCGTCAACAAGCCGCTGGAAAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCACGGGTTTATTTTGCATACTCCGCCCtn                                                                                                                                                                                                               <  1:150429/249‑2 (MQ=255)            tATTGCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGAACATAATACCAATGGTGCAATGGGGATCATCGTCAACAAGCCGCTGGAAAAGCTCAAAAGTGAAGGGATTCTGGAAAAGCTGAAGATCGCGCCGGAGCCGCGTGATGAATCAGTCCGTCTGGATAAACCGGTGAGGCTCGGCGGTCCGCTGGCTGAAGATCACGGGTTTATTTTGCATACTCCGCCCTc                                                                                                                                                                                                               <  1:235382/249‑1 (MQ=255)                     gCCTGCTCTCAAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGCACACACTACCAATCGCGCAATGGGGATCCACGCCACCAAACCGCTGGAAAATCTCAAAATTGACGGGATACTGGAAACGCTGAAGCTCACGCCCGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCACGGGTTTATTTTGCCTACTCCGCCCTCCAATTTTGCtt                                                                                                                                                                                                    >  1:42473/1‑251 (MQ=255)                         gCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGAACATAATACCAATGGTGCAATGGGGATCATCGTCAACAAGCCGCTGGAAAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCACGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTcc                                                                                                                                                                                                  <  1:122039/249‑1 (MQ=255)                         gCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATGTGCGAACATAATACCAATGGTGCAATGGGGATCATCGTCAACAAGCCGCTGGAAAATCTCAAAATTGAAGGTATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGTGTATACTCGGCGGTCCTCTTGCTGAAGATCACGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTcc                                                                                                                                                                                                  <  1:219224/249‑1 (MQ=255)                                                                                                           ncaaGCCGCTGGAAAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGACGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCACGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGAc                                                                                                                                                                  >  1:78209/2‑199 (MQ=255)                                                                                                                 cGCTGGAAAATCTGAAAAGTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGTGGTCCGCTGGCTGAAGATCACGGTTTTATTTTTCATCCCCCGCCCTCCAATTTTGCTTCGGGCATTCGCATGTCAGACAGCACGGTGATGACCAGTTCCCGCGATGTGCTGGAAACGCTCTGCACCGATAAACAACCGTCTGACGTATtg                                                                                                        <  1:226934/251‑1 (MQ=255)                                                                                                                        aaaTCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCACGGGTTTATTTTGCATACTCCGCCCTCCAATTTTg                                                                                                                                                                                                       <  1:20701/149‑1 (MQ=255)                                                                                                                                                         gAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGAGGTACGCTGGCTGAAGATCACGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGACAACTgg                                                                >  1:44493/1‑251 (MQ=255)                                                                                                                                                                           ncgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCACGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATTCTCGAt                                               >  1:47533/2‑250 (MQ=255)                                                                                                                                                                                                                       gTCCGCTGGCTGAAGATCACGGGTTTATTTGGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTTCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTGCTTGGAGAAAGGTCAACTGGGACAATAAATTCTCGATAACGCGTGGCTAACGGCCCCGGCAGATCTGAATATTCTGTTCaaa  <  1:142934/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         CATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGAACATAATACCAATGGTGCAATGGGGATCATCGTCAACAAGCCGCTGGAAAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATTCTCGATAACGCGTGGCTAACGGCCCCGGCAGATCTGAATATTCTGTTCAAA  >  NC_000913/3092949‑3093413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑