Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,072	C→T	S151F (TCT→TTT)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,072	0	C	T	91.7%	25.9 / NA	12	S151F (TCT→TTT)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (7/4);  minor base G (0/1);  total (7/5)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCA‑TGGGTTATCGGTCCACCGTTGGCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTT  >  NC_000913/77827‑78297                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   ggCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTTTCTgg                                                                                                                                                                                                                               >  1:86724/1‑251 (MQ=255) ggCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTTTCTg                                                                                                                                                                                                                                <  1:197232/250‑1 (MQ=255)                                                                    ggCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGTTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATTCGTGCGCAGCTTTTTCTGGCA‑TGGGTTATCGGTCCACCGTTGGCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTc                                                                                                                                                           <  1:226116/251‑1 (MQ=255)                                                                                                  cATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTTTCTGGCA‑TGGGTTATCGGTCCACCGTTGGCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCt                                                                                                                             >  1:12657/1‑251 (MQ=255)                                                                                                                                                 cAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTTTCTGGCA‑TGGGTTATCGGTCCACCGTTGGCCTTTATGCTGGCGTTGCATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTc                                                                                                                                  >  1:1477/1‑199 (MQ=255)                                                                                                                                                                                   cgGGAAGATGCGGATATCGCTGCTCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCCCAGCTTTGGCTGGCAGTGGGT‑ATCCGGCCACCGTGGTCCGTTATTCTAGCCTAGAAATACGGCTGTACGGGGATGTTTTCGATTGCCGCCGGGATATTCACACTc                                                                                                                                  <  1:112301/165‑1 (MQ=255)                                                                                                                                                                                    gggAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAGTCGTGCGCAGCTTTTTGTGTCA‑TGTGTTATCGGTCCTCCGTTGGCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTc                                                                                                                                  <  1:164192/164‑1 (MQ=255)                                                                                                                                                                                                      cGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTTTCTGGCA‑TGGGTTATCGGTCCCCCGTTGGCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATan                                   <  1:159413/241‑2 (MQ=255)                                                                                                                                                                                                      cGGCGCGAGAAGTGGGGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTTTCTGGCA‑TGGGTTATCGGTCCACCGTTGGCCTTTATGCTGGCGTTGAATTACGGCTTTACGGGGATGTTTTCGATTGCCGCCGGNATANNNnnacnn                                                                                                                                  >  2:159413/1‑144 (MQ=255)                                                                                                                                                                                                       gtcgcgAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCCGCTTTTTCTGGAA‑TGGGTTATCGGTCCACCGTTGGCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGCTTGCCGACGGGGTATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACgg                         >  1:228703/3‑250 (MQ=255)                                                                                                                                                                                                          ncgAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTTTCTGGCA‑TGGGTTATCGGTCCACCGTTGGCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGt                     >  1:66806/2‑251 (MQ=255)                                                                                                                                                                                                                              gCTCGGTGATGCGTGCGCAGCTTTTTCTGGCA‑TGGGTTATCGGTCCACCGTTGGCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGtt  >  1:239593/1‑250 (MQ=255)                                                                                                                                                                                                                                                     tnncTGGCA‑NNGGTTATCGGTCCACCGTTGGCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATATTCacacgc                                                                                                                                  <  2:1477/99‑3 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   GGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCA‑TGGGTTATCGGTCCACCGTTGGCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTT  >  NC_000913/77827‑78297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑