Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,204	C→T	P145P (CCG→CCA)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,204	0	C	T	100.0%	37.1 / NA	15	P145P (CCG→CCA)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/7);  total (8/7)

CGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTGGACATCTTCTTGGTCGGCTCCAGCAGCGACATTACGCGCGCGCCAGATTTCGGAATAAACGGCTCCGGCACCTTAAAGATCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTCGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCC  >  NC_000913/3512970‑3513365                                                                                                                                                                                                                                           |                                                                                                                                                                  cGCACGTTTGATTTTCTTCAATACCGATTTCGGATCTTCCAGCAGGCCGATACCGTTATTGCGATTATCGTCAGACTTGGACATCTTCTTGGTCGGCTCCAGCTGCGACCTTACGCGCGCGCCAGATTTCGGAATAAACGGCTCCGGACCCTTAAAGATATCGACATCCAGAGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACTGGTACCAGATTAGttt                                                                                                                                                   >  1:173665/1‑251 (MQ=255)                  tcACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTGGACATCTTCTTGGTCGGCTCCAGCAGCGACATTACGCGCGCGCCAGATTTCGGAATAAACGGCTCCGGCACCTTAAAGATCGCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACTGGTACCAGATTAGTTTGATACAGCAGGATg                                                                                                                                     <  1:208387/248‑1 (MQ=255)                                 gATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTGGACATTTTCTTGGTCGGCTCCAGCAGCGACATTACGCGCGCGCCAGATTTCGGAATAAACGGCTCCGGCACCTTAAAGATCTCGCCATACAGCGCGTTGAAACGATGGGCAATATCGCGGCTCGGTTCGAGGTGCTGTTTCTGGTCTTCACCCACTGGTACCAGATTAGTTTGATACAGCAGGATGTcc                                                                                                                                  >  1:40253/1‑236 (MQ=255)                                              nccGATAACGTTATTGCGATTATCGTCAGACTTGGACATCTTCTTGGTCGGCTCCAGCAGCGACATTACGCGCGCGCCAGATTTCGGAATAAACGGCTCCGGCACCTTAAAGATCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACTGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAAc                                                                                                       >  1:69231/2‑250 (MQ=255)                                                                                                                    cgcgcgcCAGATTTCGGAATAAACGGCTCCGGCACCTTAAAGATCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACTGGTACCAGATTAGTTTGATACAGCAGGATGTcn                                                                                                                                  <  1:25215/153‑2 (MQ=255)                                                                                                                         gcCAGATTTCGGAATAAACGGCTCCGGCACCTTAAAGATCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACTGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGAc                                          <  1:36549/236‑1 (MQ=255)                                                                                                                          ncAGATTTCGGAATAAACGGCTCCGGCACCTTAAAGATCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACTGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCa                                                                      >  1:151423/2‑207 (MQ=255)                                                                                                                                   nnnnnnnnnngCNNNNNCACNTTAAAGATCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACTGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCg                                                                                                                  <  2:156328/144‑1 (MQ=255)                                                                                                                                                  ngCACCTTAAAGATCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACTGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTCGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGcc  >  1:149501/2‑251 (MQ=255)                                                                                                                                                                       nanacnncattNAAACGCTGGGCTATATCGCGGCGANGTTCGAGGTGCGGTTTCTGTTCTTCACCCACNGNTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGNTAGTCAAACANACCNNCGTTNANGNTCTCGTCATAACGCGCAGATTGATCTTTANACTGCGTCATGCGACTCAGTTCGNNGANNNAGNNATAGc                  <  2:193067/205‑1 (MQ=255)                                                                                                                                                                              cgTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACTGGTACCAGATTAGTTTGATACAGCAGGATGTCAGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATaa                                                                            >  1:185015/1‑149 (MQ=255)                                                                                                                                                                              cgTNGNAACTCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTNACCCACTGGTNCCANNTTAGNTNGNTACAGCAGGAGGTCCGCTGCCATCAGCACNGGATAGTCAAACAGACCAGCGTTNNTGNNNTCNNCATaa                                                                            <  2:185015/149‑1 (MQ=255)                                                                                                                                                                                          gnnNNNTATNGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACTGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCa                                                                      <  2:151423/143‑1 (MQ=255)                                                                                                                                                                                                                        gTTTCTGGTCTTCACCCACTGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGc                                                                                                                             <  1:35790/58‑1 (MQ=255)                                                                                                                                                                                                                        gTTTCTGGACTNCACCCACTGGTACCAGATTAGTTTGATANAGCAGGATGTCNGCTGc                                                                                                                             >  2:35790/1‑58 (MQ=255)                                                                                                                                                                                                                               gTCTTNCCNCNCTNNTNCCAGNTTAGTTTGGTACAGCAGGATGTCCGCTGCCATCAGCACNGNa                                                                                                                >  2:116278/1‑64 (MQ=255)                                                                                                                                                                                                                               cgctgcacccactGGTACCAGATTAGTTCGATACAGCAGGACGTCCGCTGCCGTCCGCACCGGATAGTCAAACAGACCAGCGTTGATGTtctct                                                                                  <  1:116278/89‑2 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                  CGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTGGACATCTTCTTGGTCGGCTCCAGCAGCGACATTACGCGCGCGCCAGATTTCGGAATAAACGGCTCCGGCACCTTAAAGATCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTCGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCC  >  NC_000913/3512970‑3513365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑