Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	357,126	C→T	P319L (CCG→CTG)	codA →	cytosine/isoguanine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	357,126	0	C	T	100.0%	21.1 / NA	10	P319L (CCG→CTG)	codA	cytosine/isoguanine deaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/7);  total (3/7)

TGGGCGCGCGAGTCACCGCCAGCCACACCACGGCAATGCACTCCTATAACGGGGCGTATACCTCACGCCTGTTCCGCTTGCTGAAAATGTCCGGTATTAACTTTGTCGCCAACCCGCTGGTCAATATTCATCTGCAAGGACGTTTCGATACGTATCCAAAACGTCGCGGCATCACGCGCGTTAAAGAGATGCTGGAGTCCGGCATTAACGTCTGCTTTGGTCACGATGATGTCTTCGATCCGTGGTATCCGCTGGGAACGGCGAATATGCTGCAAGTGCTGCATATGGGGCTGCATGTTTGCCAGTTGATGGGCTACGGGCAGATTAACGATGGCCTGAATTTAATCACCCACCACAGCGCAAGGACGTTGAATTTGCAGGATTACGGCATTGCCGCCGGAAACAGCGCCAACCTGATTATCCTGCCGGCTGAAAATGGGTTTGATGCGCTGCGCCGTCAGG  >  NC_000913/356886‑357347                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                              tGGGCGCGCGAGTCACCGCCAGCCACACCACGGCAATGCACTCCTATAACGGGGCGTATACCTCACGCCTGTTCCGCTTGCTGAAAATGTCCGGTATTAACTTTGTCGCCACCCCGCTGGTCAATATTCATCTGCAAGGACGTTTCGATACGTATCCAAAACGTCGCGGCATCACGCGCGTTAAAGAGATGCTGGAGTCCGGCATTAACGTCTGCTTTGGTCACGATGATGTCTTCGATCTGTGGTATCCg                                                                                                                                                                                                                     >  1:106731/1‑251 (MQ=255)                         ccaccacGGCAATGCACTCCTATAACGGTGCGTATACCTCACGCCTGTTCCTCTTGCTGAAAATGTCCGGTATTAACTTTGTCGCCAACCCGCTGGTCAATATTCATCTGCAAGGACGTTTCGATACGTATCCAAAACGTCGCGGCATCACGCGCTTTAAAGAGAGGCTGGAGTCCGGCATTAACGTCTGCTTTGGTCACGATGATGTCTTCGATCTGTGGTATCCGCTTGGATCGTCGAATATGCTGCaa                                                                                                                                                                                             <  1:74091/250‑1 (MQ=255)                                                                                                                                                        gTATCCAAAACGTCGCGGCATCACGCGCGTTAAAGAGATGCTGGAGTCCGGCATTAACGTCTGCTTTGGTCACGATGATGTCGTCGATCTGTGGTATCCGCTGGGAACGGCGAATATGCTGCAAGGGCTGCATAGGGGGCTGCAGGTTTGCCAGTTGATGGGCTACGGGCAGATTAACGATggn                                                                                                                                 <  1:24724/184‑2 (MQ=255)                                                                                                                                                                               cgcgcgTTAAAGAGCTGCTGGCGTCCGGCATTAACGTCTGCTTTGGTCACGATGATGTCTTCGATCTGTGGTATCCGCTGGGAACGGCGAATATGCTGCAAGTGCTGCATATGGGGCTGCATGTTTGCCAGTTGATGGGCTACGGGCAGATTAACGATGGCCTGAATTTAATCACCCACCACAGCGCAAGGACGTTGAATTTGCAGGATTACGGCATTGCCGCCGGAAACAGCGCCAACCTGATTATCCTg                                       >  1:195545/1‑251 (MQ=255)                                                                                                                                                                                            gaTGCTGGAGTCCGGCATTAACGTCTGCTTTGGTCACGATGATGTCTTCGATCTGTGGTATCCGCTGGGAACGGCGAATATGCTGCAAGTGCTGCATATGGGGCTGCATGTTTGCCAGTTTATGGGCTACGGGCAGATTAACGATGGCCTGAATTTAATCACCCACCACAGCGCAAGGACGTTGAATTTGCAGGATTACGGCATTGCCGCCGGAAACAGCGCCAACCTGATTATCCTGCCGGCTGAAAAtn                          <  1:150894/251‑2 (MQ=255)                                                                                                                                                                                                 tGGAGTCCGGCATTAACGTCTGCTTTGGTCACGATGATGTCTTCGATCTGTGGTATCCGCTGGGAACGGCGAATATGCTGCAAGTGCTGCATATGGGGCTGCATGTTTGCCAGTTGATGGGCTACGGGCAGATTAACGATGGCCTGAATTTAATCACCCACCACAGCGCAAGGACGTTGAATTTGCAGGATTACGGCATTg                                                                       <  1:166864/201‑1 (MQ=255)                                                                                                                                                                                                     gTCCGGCATTAACGTCTGCTTTGGTCACGATGATGTCTTCGATCTGTGGTATCCGCTGGGAACGGCGAATATGCTGCAAGTGCTGCATATGGGGCTGCATGTTTGCCAGTTGATGGGCTACGGGCAGATTAACGATGGCCTGAATTTAATCACCCACCACAGCGCAAGGACGTTGAATTTGCAGGATTACGGCATTGCCGCCGGAAACAGCGCCAACCTGATTATCCTGCCGGCTGAAAATGGGTTTGAtg                 >  1:19890/1‑251 (MQ=255)                                                                                                                                                                                                          gCATTAACGTCTGCTTTGGTCACGATGATGTCTTCGATCTGTGGTATCCGCTGGGAACGGCGAATATGCTGCAAGTGCTGCATATGGGGCTGCATGTTTGCCAGTTGATGGGCTACGGGCAGATTAACGATGGCCTGAATTTAATCACCCACCACAGCGCAAGGACGTTGAATTTGCAGGATTACGGCATTGCCGCCGGAAACAGCGCCAACCTGATTATCCTGCCGGCTGAAAATGGGTTTGAtgcgcng            <  1:199362/251‑1 (MQ=255)                                                                                                                                                                                                                      tcTTTGGTCACGATGATGTCTTCGATCTGTGGTATCCGCTGGGAACGGCGAATATGCTGCAAGTGCTGCATATGGGGCTGCATGTTTGCCAGTGGATGGGCTACGGGCAGATTAACGATGGCCTGAATTTAATCACCCACCACAGCGCAAGGACGTTGAATTTGCAGGATTACGGCATTGCCGCCGGAAACAGCGCGAACCTGATTATCCTGCCGGCTGAAAATGGGt                       <  1:89255/227‑1 (MQ=255)                                                                                                                                                                                                                      gCTTTGGTCACGATGATGTCTTCGATCTGTGGTATCCGCTGGGAACGGCGAATATGCTGCAAGTGCTGCATATGGGGCTGCATGTTTGCCAGTTGATGGGCTACGGGCAGATTAACGATGGCCTGAATTTAATCACCCACCACAGCGCAAGGACGTTGAATTTGCAGGATTACGGCATTGCCGCCGGAAACAGCGCCAACCTGATTATCCTGCCGGCTGAAAATGGGTTTGATGCGCTGCGCCGTCAgn  <  1:150000/249‑2 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                              TGGGCGCGCGAGTCACCGCCAGCCACACCACGGCAATGCACTCCTATAACGGGGCGTATACCTCACGCCTGTTCCGCTTGCTGAAAATGTCCGGTATTAACTTTGTCGCCAACCCGCTGGTCAATATTCATCTGCAAGGACGTTTCGATACGTATCCAAAACGTCGCGGCATCACGCGCGTTAAAGAGATGCTGGAGTCCGGCATTAACGTCTGCTTTGGTCACGATGATGTCTTCGATCCGTGGTATCCGCTGGGAACGGCGAATATGCTGCAAGTGCTGCATATGGGGCTGCATGTTTGCCAGTTGATGGGCTACGGGCAGATTAACGATGGCCTGAATTTAATCACCCACCACAGCGCAAGGACGTTGAATTTGCAGGATTACGGCATTGCCGCCGGAAACAGCGCCAACCTGATTATCCTGCCGGCTGAAAATGGGTTTGATGCGCTGCGCCGTCAGG  >  NC_000913/356886‑357347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑