Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	515,644	C→T	G44R (GGG→AGG)	qmcA ←	PHB domain‑containing protein QmcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	515,644	0	C	T	100.0%	20.1 / NA	9	G44R (GGG→AGG)	qmcA	PHB domain‑containing protein QmcA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

GGTACGGATGTTAGTCATGGTCAGGTTGATGATCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGATATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGA  >  NC_000913/515420‑515875                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        ggTACGGATGTGAATCATGGTCAGGTTGATGATCGCCAACTCCAGATTGCTGAATTCATAAGCCGCGCGCGGCGCGTCAATCCCCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATACCTTCCTGGGAAGGGAGATCGAGCACTTGCGCCATCAGATTGAGCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCTCGGCTGTAACGTTTTGGTATAGCGAc                                                                                                                                                                                                               <  1:177085/251‑1 (MQ=255)                                           cAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGATATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCTCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGtt                                                                                                                                                                                                      >  1:165105/1‑218 (MQ=255)                                                                          gcgTCAATCACTTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCATGGGTAGGGATATCGAGCACTTGCTCCATAATATTGATCTTGCGACCAATGAGATCCATAAACGGCACCACCAGACTGAGCCTCGGCTGTAACGTTTTGGGATAGCGACCAAAGCGTTCTACTGTCAACTGATAGCCCTGCGGTCCGATTTTGACACCCGCGCAGACAATGACCAGCGCGAc                                                                                                                                      >  1:161282/1‑251 (MQ=255)                                                                                                               tAACGTTGGCGTTATGTTTCGAGATAACTGCCTGGGAAGGGATATCGAGCACTTGCTCCATCATAGTGATCGTGCGACCAATGCTGTCCATAATCGGCACCACCAGACTGAGCTTCGGCTGTAGCTTTTTGGTATAGTGACCATAGCGTTCTACTTGCCGTTGATAGCCCTGCGGTACGGTTTTGTCGCCCGCCCCGACAATGACCAGCTCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGaaa                                                                                                  <  1:118248/250‑1 (MQ=255)                                                                                                                                          cTTCCTGGGAAGGGATATCGAGCACTTGCTCCATCATATCGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCTCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATc                                                                        >  1:128493/1‑249 (MQ=255)                                                                                                                                                                                 tgagcTTGCGACCAATGGGATCCATAGACGGCAGCGCCATACTGAGCCTCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTc                                                                                                                                                                                              <  1:241718/88‑1 (MQ=255)                                                                                                                                                                                                           aaCGGCACCACCAGACTGAGCCTCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCAtn                                                                                                       <  1:151697/153‑2 (MQ=255)                                                                                                                                                                                                           aaCGGCACCACCAGACTGAGCCTCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAANCggnnnnn                                                                                                                  >  2:151697/1‑137 (MQ=255)                                                                                                                                                                                                                caccacCAGACTGAGCCTCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa  >  1:10519/1‑249 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        GGTACGGATGTTAGTCATGGTCAGGTTGATGATCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGATATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGA  >  NC_000913/515420‑515875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑