Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,512,791	C→T	G283D (GGT→GAT)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,512,791	0	C	T	100.0%	24.8 / NA	12	G283D (GGT→GAT)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/5);  total (7/5)

CCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCAATCGCTTCGTACACCGCTTTTAGCGTACGGGAAGCGTGCGCGCTGGCTTTTTCCGCGCCATCTTTCATCACCTGTTGCAGGAAGGCTTCATCGTTGCGGAAACGGTGATAGCGTTCCTGCAATTCAGTCAGCATACCGGAAACGGCATCAGCCACTTCACCTTTCAGATGACCATACATCTTGCCTTCGAACTGTTTTTCCAGTTCTGGGATGCTCTGGCCCGTTACCGCTGAAAGGATATCCAACAGGTTGGAAACGCCCGCTTTGTTCTGCACATCGTAGCGAACTACCGGCGGCTCGTCGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCG  >  NC_000913/3512590‑3513001                                                                                                                                                                                                          |                                                                                                                                                                                                                   ccgcATCCGGCATGAACACAGCGCAATTTGCCAGCACTAGTGAATTACGGCTTCGCCACAAAACCAATCGCTTCGTACACCGCTTTTAGCGTACGGGAAGCGTGCGCGCTGGCTTTTTCCGCGCCATCTTTCATCACCTGTTGCAGGAAGGCTTCATCGTTGCGGAAACGGTGATAGCGTTCCTGCAATTCAGTCAGCATATCGGAAACGGCATCAGCCACTTCACCTTTCAGATGACCATACATCTTGcc                                                                                                                                                                   >  1:214711/1‑251 (MQ=255)        cGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCAATCGCTTCGTACACCGCTTTTAGCGTACGGGAAGCGTGCGCGCTGGCTTTTTCCGCGCCATCTTTCATCACCTGTTGCAGGAAGGCTTCATCGTTGCGGAAACGGTGATAGCGTTCCTGCAATTCAGTCAGCATATCGGAAACGGCATCAGCCACTTCACCTTTCAGATGACCATACATCTTGCCTTCGAAc                                                                                                                                                            >  1:179886/1‑251 (MQ=255)                          aTTTGCCATCAATAGTGAATTACGGCTTCGCCACAAAACCAATCGCTTCGTACACCTCTTTTAGCGTACGGGAAGCGTGCGCGCTGGCTTTTTCCGCGCCCGCTTTCATCACCTGTTGCAGGAATGCTTCATCGTGTCGGAAACGGTGATAGCGTGCCTCCAATTCAGTCATCATATCGGTAACTGCATCATACACTCCTCCTTTCAGATGGCCATACATCTTGCCTTCGAACGGTTTTTCCCGTTCTggg                                                                                                                                          <  1:186346/251‑1 (MQ=255)                                                     tCGCCACAAAACCAATCGCTTCGTACACCGCTTTTAGCGTACGGGAAGCGTGCGCGCTGGCTTTTTCCGCGCCATCTTTCATCACCTGTTGCAGGAAGGCTTCATCGTTGCGGAAACGGTGATAGCGTTCCTGCAATTCAGTCAGCATATCGGAAACGGCATCAGCCACTTCACCTTTCAGATGACCATACATCTTGCCTTCGAACTGTTTTTCCAGTTCTGGGATGCTCTGGCCCGTTACCGCTGaaan                                                                                                                <  1:154502/250‑2 (MQ=255)                                                               aCCAATCGCTTCGTACACCGCTTTTAGCGTACGGGAAGCGTGCGCGCTGGCTTTTTCCGCGCCATCTTTCATCACCTATTGCAGGAAGGCTTCATCGTTGCGGAAACGGTGATAGCGTTCCTGCAATTCAGTCAGCATATCGGAAACGGCATCAGCCACTTCACCTTTCAGATGACCATACATCTTGCCTTCGAACTGTTTTTCCa                                                                                                                                                  <  1:58588/206‑1 (MQ=255)                                                                  aaTCGCTTCGTACACCGCTTTTAGCGNACGGGANNCGNGCGCGCTGGCTTTTTCCGCNCCNNNTNNNANCACCTNTTNNAGGAAGGCTNCATCGTTGCGGAAACGGTGATAGCGTTCCTGCACTTCAGTCAGCATATCGGAANCGGCATCAGNNACTTCACCTTTCAGATGACCANACANNTNNCNtttnaa                                                                                                                                                             >  2:56058/1‑188 (MQ=255)                                                                                       tAGCGTACGGGAAGCGTGCGCGCTGGCTTTTTCCGCGCCATCTTTCATCCCCTGTTGCAGGAAGGCTTCATCGTTGCGGAAACGGTGATAGCGTTCCTGCAATTCAGTCAGCATATCGGAAACGGCATCAGCCACTTCACCTTTCAGATGACCATACATCTTGCCTTCGAACTGTTTTTCCAGTTCTGGGATGCTCTGGCCCGTTACCGCTGAAAGGATATCCAACATGTTGGAAACGCCCGCTTTGTTc                                                                              <  1:56058/250‑1 (MQ=255)                                                                                             aCGGGAAGCGTGCGCGCTGGCTTTTTCCGGGCCATCTTTCATCACCTGTTGCAGGAAGGCTTCATCGTTGCGGAAAAGGTGATAGCGTTCCTGCAATTCAATCAGCATATCGGAAACGGCATCAGCCACTTCACCTTTCAGATGACCATACATCTTGCCTTCGAACTGTTTTTCCAGTTCTGGGATGCTCTGGCCCGTTACCGCTGAAAGGATATCCAACAGGTTGGAAACGCCCGCTTTGTTCTGCACAt                                                                       >  1:200808/1‑251 (MQ=255)                                                                                                                            ccATCTTTCATCACCTGTCGCAGGAAGGCTTCATCGTTGCGGAAACGGTGATAGCGTTCCTGCAATTCAGTCAGCATATCGGAAACGGCATCAGCCACTTCACCTTTCAGATGACAATACATCTTGCCTTCGAACTGTTTTTCCAGTTCTGGGATGCTCTGGCCCGTTACCGCTGAAAGGATATCCCACAGGTTGGAAACGCCCGCTTTGTTCTGCACATCGTAGCGAACTACCGGCGGCTCGTCGGAGTc                                        >  1:193067/1‑251 (MQ=255)                                                                                                                                                     aGGCTTCATCGTTGCGGAAACGGTGATAGCGTTCCTGCAATTCAGTCAGCATATCGGAAACGGCATCAGCCACTTCACCTTTCAGATGACCATACATCTTGCCTTCGAACTGTTTTTCCAGTTCTGGGATGCTCTGGCCCGTTACCGCTGAAAGGATATCCAACAGGTTGGAAACGCCCGCTTTGTTCTGCACATCGTAGCGAACTACCGGCGGCTCGTCGGAGTCAGTGACCGCACGTTTGATTttcttc               <  1:89446/251‑1 (MQ=255)                                                                                                                                                                  gCGGAAACGGTGATAGAGTTCCTGCAATTCAGTCAGCATATCGGAAACGGCATCAGCCACTTCACCTTTCAGATGACCATACATCTTGCCTTCGAACTGTTTTTCCAGTTCTGGGATGCTCTGGCCCGTTACCGCTGAAAGGATATCCAACAGGTTGGAAACGCCCGCTTTGTTCTGCACATCGTAGCGAACTACCGGCGGCTCGTCGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCg  >  1:207750/1‑251 (MQ=255)                                                                                                                                                                   cGGAAACGGTGATAGCGTTCCTGCAATTCAGTCAGCATATCGGAAACGGCATCAGCCACTTCACCTTTc                                                                                                                                                                                       >  1:36524/1‑69 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                   CCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCAATCGCTTCGTACACCGCTTTTAGCGTACGGGAAGCGTGCGCGCTGGCTTTTTCCGCGCCATCTTTCATCACCTGTTGCAGGAAGGCTTCATCGTTGCGGAAACGGTGATAGCGTTCCTGCAATTCAGTCAGCATACCGGAAACGGCATCAGCCACTTCACCTTTCAGATGACCATACATCTTGCCTTCGAACTGTTTTTCCAGTTCTGGGATGCTCTGGCCCGTTACCGCTGAAAGGATATCCAACAGGTTGGAAACGCCCGCTTTGTTCTGCACATCGTAGCGAACTACCGGCGGCTCGTCGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCG  >  NC_000913/3512590‑3513001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑