Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	862,842	C→T	G224S (GGC→AGC)	ybiY ←	putative pyruvate formate‑lyase activating enzyme YbiY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	862,842	0	C	T	100.0%	23.8 / NA	11	G224S (GGC→AGC)	ybiY	putative pyruvate formate‑lyase activating enzyme YbiY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/4);  total (7/4)

GCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCTCGTCGGCGGCAAAATCGGTAATGGCTTTTACAGAGGTTTCGTCGGCATTAAAGCCCTGAATCAGCGGCACGCGGATAATGATTTTTTTGCCCGCTGCGGCGAGTTTTTTCAGGTTATCCAGCACTCTGGCGGCGTTACCGTCGGTCCACTGTTTAAACGGCGCGTCGGCAACGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGC  >  NC_000913/862628‑863069                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    gcgGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGTGCAGTTCTGGCGCATCAAGCGGTTTTTCCGGGGAGTCATAGGGCAGGTTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCTCGTCGGCGGCAAAATCGGTAATGGATTTTACAGAGGTTTCGTCGGCATTAAAGCTCTGAATCAGCGGCCCGCGGATAATGATTTTTTTGcc                                                                                                                                                                                                 >  1:56867/1‑251 (MQ=255)       aaCCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCTCGTCGGCGGCAAAATCGGTAATGGCTTTTACAGAGGTTTCGTCTGCATTAAAGCTCTGAATCAGCGGCACGCGGATAATGATTTTTTTGCCCGCtn                                                                                                                                                                                            <  1:203960/250‑2 (MQ=255)                      gcaTACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCTCGTCGGCGGCAAAATCGGTAATGGCTTTTACAGAGGTTTCGTCGGCATTAAAGCTCTGAATCAGCGGCACGCGGATAATGATTTTTTTGCCCGCTGCGGCGAGTTTTTTc                                                                                                                                                                              >  1:210719/1‑249 (MQ=255)                                                              gCGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCTCGTCGGCGGCAAAATCGGTAATGGCTTTTACAGAGGTTTCGTCGGCATTAAAGCTCTGAATCAGCGGCACGCGGATAATGATTTTTTTGCCCGCTGCGGCGAGTTTTTTCAGGTTATCCAGCACTCTggc                                                                                                                                                          >  1:15608/1‑229 (MQ=255)                                                                                                 aagtGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCTCGTCGGCGGCAAAATCGGTAATGGCTTTTACAGAGGTTTCGTCGGCATTAAAGCTCTGAATCAGCGGCACGCGGATAATGATTTTTTTGCCCGCTGCGGCGAGTTTGTTCAGGTTATCCAGCACTCTGGCGGCGTTACCGTCGGTCCACTGTTTAAACGGCGCGTCGGCAACGTGTTTTAAATCgn                                                                                                  <  1:48171/250‑2 (MQ=255)                                                                                                                        cGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGGGCAGCTCGTCGGCGGCAAAATCGGTAATGGCTTTTACAGAGGTTTCGTCGGCATTAAAGCTCTGAATCAGCgn                                                                                                                                                                                                                         <  1:24805/108‑2 (MQ=255)                                                                                                                                                        ngTGCAGCTCGTCGGCGGCAAAATCGGTAATGGCTTTTACAGAGGTTTCGTCGGCCTTAAAGCTCTGAATCAGCGGCACGCGGATACTGATTTTTTTGCCCGCTGCGGCGAGTTTTTTCAGGTTATCCAGCCCTCTGGCGGCGTTACCGTCGGTCCACTGTTTAAACGGCGCGTCGGCAACGTGTTTTAAATCGGCACGAAACAGATCGATATAGGGCAGAGCAggg                                                                  >  1:54978/2‑227 (MQ=255)                                                                                                                                                                        ggcAAAATCGGTAATGGCTTTTACAGAGGTTTCGTCGGCATTAAAGCTCTGAATCAGCGGCACGCGGATAATGTTTTTTTTGCCCGCTgcggc                                                                                                                                                                                        >  1:168670/1‑93 (MQ=255)                                                                                                                                                                        ggcAAAATCGGTAATGGCTTTTAAAGAGGTTTCGTCGGCATTAAAGCTCTGAATCAGCGGCCCGCGGATAATGATTTTTTTGCCCGCTGCGGCGGGTTTTTTCAGGTTATCCACCACTCTGGCGGCGTTACCGCCGGTCCACTGTTTAAAGGGCGCGTCGGCCACGTGTTTTAAATCGGCAAGAAAc                                                                                          >  1:221019/1‑187 (MQ=255)                                                                                                                                                                                                agagGGTTCGTCGGCATTAAAGCTCTGAATCAGCGGCCCGCGGATAATGATTTTTTTGCCCGCTGCGGCGAGTTTTTTCAGGTTATCCAGCACTCTGGCGGCGTTACCGTCGGTCCACTGTTTAAACGGCGCGTCGGCAACGTGTTTGAAATCGGCAAGAAACAGATCGATATAGGGCAGATAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTGTCTACCGCAGTATGAATGCCTGCCTCGtggc  <  1:86114/251‑1 (MQ=255)                                                                                                                                                                                                             ncATTAAAGCTCTGAATCAGCGGCACGCGGCTAATGATTTTTTTGCCCGCTGCGGCGAGTTTTTTCAGGTTATCCAGCACTCTGGCGGCGTTACCGTCGGTCCACTGTTTAAACGGCGCGTCGGCAACGTGTTTTAAATCGGCAAGAACCAGCTCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCCGTATg                   >  1:69774/2‑221 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    GCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCTCGTCGGCGGCAAAATCGGTAATGGCTTTTACAGAGGTTTCGTCGGCATTAAAGCCCTGAATCAGCGGCACGCGGATAATGATTTTTTTGCCCGCTGCGGCGAGTTTTTTCAGGTTATCCAGCACTCTGGCGGCGTTACCGTCGGTCCACTGTTTAAACGGCGCGTCGGCAACGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGC  >  NC_000913/862628‑863069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑