Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,087,272	G→A	Q189Q (CAG→CAA)	metK →	methionine adenosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,087,272	0	G	A	90.0%	17.4 / ‑5.9	10	Q189Q (CAG→CAA)	metK	methionine adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (3/6);  total (3/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGGCGCGGGTGACCAGGGTCTGATGTTTGGCTACGCAACTAATGAAACCGACGTGCTGATGCCAGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCG  >  NC_000913/3087048‑3087475                                                                                                                                                                                                                                 |                                                                                                                                                                                                            cAGGGCGCGGGTGACCAGGGTCTGATGTTTGGCGACGCAACTAATGAAACCGACGTGCTGAGGCCAGCCCCTAGCCCCTATGCACACCGTCTGGTACAGCGGCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGTACGCGAAAAGCCATGTGACTTTTCATTATGACGACGGCAAAATCGTTGGTATCGGTGCTGTCGTGCTTTCCACTCAACACTCTGAAGAGATCGACCAGAAATc                                                                                                                                                                                   <  1:162441/251‑1 (MQ=255)                                    cGCAACTAATGAAACCGACGTGCTGATGCCAGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTTGCTGCGCCCGGACGCGAAAAGCCAGGTGTCTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTGCCACTCAACACTCTGAAGAGATGGACCAGAAATTGCTGCAAGAAGCGGTGATGGAAGAGATCATCAAGc                                                                                                                                                <  1:81864/251‑1 (MQ=255)                                                    gACGTGCTGATGCCAGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGCATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAACACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTg                                                                                                                                 >  1:205970/1‑250 (MQ=255)                                                                                                                  gCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGGATCGATGCTGTCGTGCGTTCCACTCAACGCTCTGAGGAGATCGACCAGAAAGCGCTGCAAGATGCGGTAATGGAAGAGATCATCAAGTCAGTGCTGCCCGCTGAATAGCTGACTGCTGCCACCAAAGTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGc                                                                   <  1:122392/250‑1 (MQ=255)                                                                                                                         aaCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTTTCGTGCTTTCCACTCAACACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATggg                                                           <  1:15865/251‑1 (MQ=255)                                                                                                                             gCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGGCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGAc                                                       <  1:130398/251‑1 (MQ=255)                                                                                                                                                                  ggTGACTTTTCAGTATGACGACGGCAAAATCGTTGCTATCGATGCTGTCGTGCTTTCCACTCAACACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGt                                                                                                                                                                    >  1:94308/1‑105 (MQ=255)                                                                                                                                                                      cgtgtactcttccgatctggCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAACACTCGGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTGATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCTGTCGTTTCGTTATCGGTGGCGCAATGGGTGACTGCGGTCTGACTTGGCGTAAAATTATCGGTGATACCTAcg               <  1:82922/232‑1 (MQ=255)                                                                                                                                                                                  gacgacGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAACACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCAAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGcgcg  >  1:111973/1‑251 (MQ=255)                                                                                                                                                                                                                           cACTCAACACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATg                                                             <  1:5751/151‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                            CAGGGCGCGGGTGACCAGGGTCTGATGTTTGGCTACGCAACTAATGAAACCGACGTGCTGATGCCAGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCG  >  NC_000913/3087048‑3087475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑