Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,723,212	G→A	Q162Q (CAG→CAA)	pssA →	phosphatidylserine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,723,212	0	G	A	100.0%	19.6 / NA	8	Q162Q (CAG→CAA)	pssA	phosphatidylserine synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/5);  total (3/5)

CGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTTTACCTGCATCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGTCTGGATGATGTTAATCGGCCAAAAAGCCCGGAAATCAAGAACGATATTCGTCTGTTCCGCCAGGAGCTGCGTGATGC  >  NC_000913/2723012‑2723400                                                                                                                                                                                                         |                                                                                                                                                                                             cGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTTTACCTGCATCAACACGATAAATATCGCTACGACCGTTATCATCTGATccgaaaccgta                                                                                                                                                >  1:137134/1‑247 (MQ=255)       ggCGCTGCGGCATCTAACACTAACGCTGACTGGTTCTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTTTACCTGCATCAACACGATAAATATCGCTACGACCGGTATCATCTGATCCGTAACCGTAAGATGTCAg                                                                                                                                       <  1:216009/250‑1 (MQ=255)               ngCATCTAACACTAACGCTGACTGGTACTGCCGCATGCCGCAGGAAAATTCGGGCGAAGCTGTTCAGGTTTATGGCGTTCCCATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTCTAGCGGTGCAAGCCTGAACGATGTTTACCTGCATCAACACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGt                                                                                                                              >  1:24124/2‑251 (MQ=255)                            aaCGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTTTACCTGCATCAACACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGttn                                                                                                                  <  1:157162/250‑2 (MQ=255)                                          cTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTTTACCTGCATCAACACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATggn                                                                                                                     <  1:159351/233‑2 (MQ=255)                                                                                                                   cTTGGTGGTCTGCACTTTAAATGCTATATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTTTACCTGCATCAACACGATAAATATCGCTACGCCCGTTatc                                                                                                                                                                  <  1:165265/115‑1 (MQ=255)                                                                                                                                            ttATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTTTACCTGCATCAACACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCTTTAATCGTCTGGATGATGTTAATCGGCCAAAAAGCCCGGAAATCAAGAACGATATTCGTCTGTTCCGCCAGTAGCTGCGTGATGc  <  1:71440/250‑1 (MQ=255)                                                                                                                                                                                                     aTCAACACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGCATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGTCTGgat                                                                         >  1:90482/1‑122 (MQ=255)                                                                                                                                                                                                         |                                                                                                                                                                                             CGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTTTACCTGCATCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGTCTGGATGATGTTAATCGGCCAAAAAGCCCGGAAATCAAGAACGATATTCGTCTGTTCCGCCAGGAGCTGCGTGATGC  >  NC_000913/2723012‑2723400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑