Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,227,114	C→T	T294I (ACC→ATC)	ygjI →	putative transporter YgjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,227,114	0	C	T	92.9%	26.7 / NA	15	T294I (ACC→ATC)	ygjI	putative transporter YgjI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (5/8);  minor base G (0/1);  total (5/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

ATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACC  >  NC_000913/3226984‑3227340                                                                                                                                   |                                                                                                                                                                                                                                   aTTAACGTCTTCGCCATCAGCAAAGAGTTGAAATTCACCGGCGGCTCGGTGCAGTTATTCCACGTCAGGGCGGCGTATTTTGATTTACCGTAATCGTTGATGAATCTCTTTGCCGGTTTGGGGTCCTTTAACTCAACGTTCTTGACCCTGATGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCgg                                                                                                                                                                <  1:230005/199‑1 (MQ=255)                   ngcaAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGaa                                                                                                                              >  1:157840/2‑215 (MQ=255)                        nnagacgcncnncagatntggNTCGGTGCAGGTAGTCCACGGCATGGAGGCGTATTTTGGTCTATCGGAAGCGTTTATGAATCGCTTTGTCGGTCTGGTGGCCTGTAGCGCGAGGTTCGGTTCCCTGNGGANTTGTNCCNCAACGCCTGTGAAAANTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGNNGNNNTNAACGaaaa                                                                                                                        <  2:183039/197‑1 (MQ=255)                        agagTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTcn                                                                                     <  1:67143/251‑2 (MQ=255)                                      ncggcggATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGa                                                                                                                                    >  1:25993/2‑190 (MQ=255)                                           ggATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGCATAGCTTTGTCGGACTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAAAGCCGGTGAAAATTTTCTTCTCCGAAATACCGGAAGGCATCTTTGGTAAGAAAAACGTCGAACTGAACGaaaa                                                                                                                        >  1:183039/1‑197 (MQ=255)                                            gATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTATCTCGATGGTCGGTTCCCGGCGGATGTGGGCCGCAACGCCGGTGAAAATTTTCTTCGCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACg                                                                                                                            <  1:38756/192‑1 (MQ=255)                                                     aGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTNTCCNNNNNccnnnnnnnnnn                                                                                                                                                         >  2:154253/1‑144 (MQ=255)                                                     aGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTcn                                                                            <  1:154253/231‑2 (MQ=255)                                                                      gcggcgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTGCTGCTCCTAAATCCCGGAAGGCTGCTTTGGTAATATAACCGTCGAACTGAACGAAAACGGCTTTCCGGCGCTCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTTGGTTCCAATAc                                       <  1:40461/251‑1 (MQ=255)                                                                                nnnnnnnnnnggNNNNNTTGNTGAATCGCTTTGTCGGTCTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGaa                                                                                                                              <  2:157840/144‑1 (MQ=255)                                                                                                        agcGCTTTGTCGGTCTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatatn     <  1:63657/249‑2 (MQ=255)                                                                                                           gCTTTGTCGGTCTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAcc  <  1:41640/251‑1 (MQ=255)                                                                                                                   ggTCTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAAAGCCGGTGaaa                                                                                                                                                                                      >  1:175782/1‑63 (MQ=255)                                                                                                                   ggNCTGGTGTCCTTTATCGCGATGTTCGGTTCCCTGCTGATGTGGACNNCNNNGNCGGTGaaa                                                                                                                                                                                      <  2:175782/63‑1 (MQ=255)                                                                                                                                   |                                                                                                                                                                                                                                   ATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACC  >  NC_000913/3226984‑3227340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑