Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	510,345	C→T	M345I (ATG→ATA)	copA ←	Cu(+) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	510,345	0	C	T	100.0%	47.4 / NA	18	M345I (ATG→ATA)	copA	Cu(+) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/7);  total (11/7)

GCTGCTCTGGGCCTGGCGCACCATGCGAATGATTCGTGACAGCGTAGTATGGCTGCCAACCGCACTGGCACGAAACAGCACACTGCCGTCCTGTACCACTGTCCCGGCATGGACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAGATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAAT  >  NC_000913/510105‑510583                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               nctgctCTGGGCCTGGCGCACCATGCGAATGATTCGTGACAGCGTAGTATGGCTGCCAACCGCACTGGCACGAAACAGCACACTGCCGTCCTGTACCACTGTCCCGGCATGGACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCt                                                                                                                                                                                                                                      >  1:160370/2‑251 (MQ=255)                                                   ggCTGCCAACCGCACTGGCACGAAACAGCACACTGCCGTCATGTACCACTGTACAATCCTGGACGCTATCGCCTCCGCCTTTTTGCTGCGGGCTTCGTTCGCCCGTCAGCATCGCTTCATCCAGCCATCCTTCGCCCTGGGTACTCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCTGCACt                                                                                                                                                                                                                                 >  1:44032/1‑206 (MQ=255)                                                       gCCAACCGCACTGGCACGAAACAGCACACTGCCGTCCTGTACCACTGTCCCGGCATGGACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCg                                                                                                                                                                                >  1:13747/1‑251 (MQ=255)                                                                           aCAGCACACTGACGTCCTGTACCACTGTCCCGGCATGGACGCTATCGCCTTCGCCTTTTTGCTGCGGGCTTGGTTAGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTCATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCCATATACCTGGCTGCACTTCTGcc                                                                                                                                                                                                                           >  1:36982/1‑188 (MQ=255)                                                                                        gTCCTGTACCACGGTCCCGGCATGGACGCTATCGCCGTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCGTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTc                                                                                                                                                                                                          <  1:99475/192‑1 (MQ=255)                                                                                                             aTGGACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGTCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTg                                                                                                                          <  1:192194/251‑1 (MQ=255)                                                                                                              nngANNNNNTCGNCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCTGCAc                                                                                                                                                                                                                                  <  2:160370/144‑1 (MQ=255)                                                                                                                     tagcgccttcgccttTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTc                                                                                                                                                                                                          <  1:120157/160‑1 (MQ=255)                                                                                                                                             ggATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAATAGACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGTTTAACTTTTCCAGCGTCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGAtn                                                                                          <  1:63688/251‑2 (MQ=255)                                                                                                                                                               gNATCGCTTCATACAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTTAATCGAGTAACTTTTCCCGCGCCTTAGAAGAACGCTgg                                                                                                                              >  1:198649/1‑197 (MQ=255)                                                                                                                                                                              gCCATGCTTCGCCCTGGGTACTCTCGCCATCTACCGGCACGTGATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGCACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGtaata                                                          >  1:58758/1‑250 (MQ=255)                                                                                                                                                                                cNTGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAgg                                                                                                                                                                                       >  1:198757/1‑123 (MQ=255)                                                                                                                                                                                                               ccGGCACGCGATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCCCCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCATCGCCTTATAAGAACGCTGGCGTGCGCGCGCTTCCATCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTTATAAAGATGTCGCGCTTCCATCGGGAACCACTGCGGc                        <  1:222095/251‑1 (MQ=255)                                                                                                                                                                                                                       ngATCGCCGGTCGTCAGGCGCAGCAATATACCTGGCTGCGCTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTCAATCGAGTAACTTTTCCAGCGCCTTAGAAGACCGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGATTCGTAATAAAGATGTCGCGCTTCCATCgggg                                   >  1:55404/2‑231 (MQ=255)                                                                                                                                                                                                                                   gtcATGCGCAGCAATATACCTGGCTTCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGGCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGTCCGAGATTGAGCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAGATGTcgc                                                <  1:39286/207‑1 (MQ=255)                                                                                                                                                                                                                                   gtcAGGCGCAGCAATATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAGATGTCGCGCTTCCATCGGGa                                   >  1:195585/1‑220 (MQ=255)                                                                                                                                                                                                                                     cAGGCGCCGCAATATCCCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAGATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAAt  >  1:202319/1‑251 (MQ=255)                                                                                                                                                                                                                                       ggCGCAGCAATATACCTGGCTGCACTTCTGCCAGCGGCACGCTTTTTTAACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCCTATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAGATGTCGCGCTTCCATCGGGAACCACTGc                           >  1:214207/1‑224 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               GCTGCTCTGGGCCTGGCGCACCATGCGAATGATTCGTGACAGCGTAGTATGGCTGCCAACCGCACTGGCACGAAACAGCACACTGCCGTCCTGTACCACTGTCCCGGCATGGACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAGATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAAT  >  NC_000913/510105‑510583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑