Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	155,807	C→T	S132N (AGC→AAC)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	155,807	0	C	T	100.0%	20.1 / NA	9	S132N (AGC→AAC)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/3);  total (6/3)

CGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATC  >  NC_000913/155602‑156018                                                                                                                                                                                                              |                                                                                                                                                                                                                    cGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTTTGCGAAATGCCAGTTGCAGCAGGTTTTGATTCGCGATCTTTTCTGCATCTGGTTTTGGTGGAACTTCCa                                                                                                                                                                        <  1:81694/251‑1 (MQ=255)  ggATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGATACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCGTACCTCCTGAACCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGTATTTCCCTTCAATCCGTCCGGGCGATAGAAAAGTTTTAGTCGTGGGCGAAATGCCAGTTGCAGTAGGTTTTGATTCGCGACCTTTTCTGCATCTGGTTTtggtg                                                                                                                                                                                 <  1:162994/241‑1 (MQ=255)   gATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGTTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGt                                                                                                                                                                      >  1:6411/1‑251 (MQ=255)                                ccacTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCAGAACCAATTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGTTTTGATTCGCGACCTTTTCTGCATCTGGTTTtggtg                                                                                                                                                                                 >  1:142204/1‑211 (MQ=255)                                                cGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGTTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTg                                                                                                                         >  1:20465/1‑251 (MQ=255)                                                             tGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGTTTTGATTCGCGACCTTTTCTGCATCTGGTTGTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCGCTGTCTTTAGGCAGTGAGGt                                                                                                                          <  1:234089/237‑1 (MQ=255)                                                                                                                                 cTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCGGCAGGTTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAAGTTACCGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGACCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACt                                        >  1:109161/1‑251 (MQ=255)                                                                                                                                                         catccGGGCGATAGAAAAGTTTTATACGTGTGCGAAACGCCAGTTGCAGCAGGTTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGt                                                                                                                                                                      >  1:118853/1‑101 (MQ=255)                                                                                                                                                                                                ccAGTTGCAGCAGGTTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACAAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATc  >  1:209046/1‑226 (MQ=255)                                                                                                                                                                                                     tNNAGCAGGNNTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAg                                                                                                                            <  2:6411/99‑1 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                    CGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACCAGAACCACTTCAGGGCTAACGGGGCTTCAGAGGGATTTCCCTTCAATCCATCCGGGCGATAGAAAAGTTTTATACGTGTGCGAAATGCCAGTTGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATC  >  NC_000913/155602‑156018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑