Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,328,745	G→A	R351R (CGC→CGT)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,328,745	0	G	A	100.0%	14.2 / NA	8	R351R (CGC→CGT)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

GCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAA  >  NC_000913/2328525‑2328923                                                                                                                                                                                                                             |                                                                                                                                                                                   cccccGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCGAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTTGCGCTGCTGTCTTAGATGGTCAGTCAATCGCCTTCTCGAGTTGCGTTTTGCTGTAAAAAAGTACGAATCGCCACGGTCATGGCTTTCGCTGcc                                                                                                                                                      <  1:143752/250‑1 (MQ=255)                                                                       nggATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGCAGCAGAAAAACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTACGAATCGCCACGGGCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCCGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACt                                                                                >  1:204438/2‑251 (MQ=255)                                                                                                              cATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTACGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGc                                                        <  1:35368/236‑1 (MQ=255)                                                                                                                         nccccGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGCTGGTCACGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAACAGTACGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGCTCCAGAACTCTGGCAACGTCCTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTg                                                                  >  1:32778/2‑215 (MQ=255)                                                                                                                                              cAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTAAGGCAATCGCCTTCCCGCTTGGCGTTTTGCTGTAAAAAAGTACGCATCGACCCGGTCTTGGCTTTCGc                                                                                                                                                          >  1:192640/1‑106 (MQ=255)                                                                                                                                                      cGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTACGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaa  >  1:141915/1‑250 (MQ=255)                                                                                                                                                                         cGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTACGAATCGCCACGGTCATGGCTGTCGCTGCCGCAGGGGGCGTACGTGTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCg                                                         <  1:82224/176‑1 (MQ=255)                                                                                                                                                                                                              ttGCTGTAAAAAAGTACGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGc                                                                 <  1:146837/131‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                   GCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAA  >  NC_000913/2328525‑2328923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑