Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,912,335	G→A	T81I (ACT→ATT)	htpX ←	protease HtpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,912,335	0	G	A	100.0%	12.1 / NA	7	T81I (ACT→ATT)	htpX	protease HtpX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/2);  total (5/2)

TTTCGTGAGCAATTACCGCCTCGGCTTCATCCGGGCTCATGTTCTGCAGCAAACCGGTGCTGACAGCAACCAGAGAGGCATCACGGCGCGCACCGGTTGCAAAAGCGTTGATGTCCGGCGCATGGTAGATAGC‑CACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGTTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGAAACGAAGGAACCACCAAAACCGAACAGCAAGGCCATGATCATCAGCCCCTGAACGCTGCTCGACTGTATCCCTGTCAGGCTCAGTACCAGCCCGAAAA  >  NC_000913/1912156‑1912527                                                                                                                                                                                     |                                                                                                                                                                                                 catCGTGAGCAATTACCGCCTCGGCTTCATCCGGGCTCATGTTCTGCAGCAAACCGGTGCTGACAGCAACCAGAGAGGCATCACGGCGCGCACCGGTTGCAAAAGCGTTGATGTCCGGCGCATGGTAGATAGC‑CACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAATATTGACCAGCCAACGTTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGc                                                                                                                            <  1:84706/248‑1 (MQ=255)                                              gcagcaAACCGGTGCTGACAGCAACCAGAGAGGCATCACGGCGCGCACCGGTTGCAAAAGAGTTGATGTACGGCGCATGGTAGATAGC‑CACTTGCGGCATAGCGATCCACGCCTGACGAGCCTGGGTTGCTACAATATTGACCAGCCAACGTTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGAAACGAAGGAACCACCAAAACCg                                                                              >  1:168550/1‑251 (MQ=255)                                                                                               ggTTGCAAAAGCGTTGATGTCCGGCGCATGGTAGATAGC‑CACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAATATTGACCAGCCAACGTTCCCTTGCGTTACGCGGTTGCTCGATCTCTTCCCCGCCAACAGATCGTAATTCCATCCATTTGGACATCAGAAGCGAAACGAAGGAACCACCAAAACCGAACAGCAAGGCCATGATCATCAGCCCCTGAACGCTGCTCGACTGTATcn                             <  1:25315/251‑2 (MQ=255)                                                                                                    cAAAAGCGTTTATGTCCGGCGCATGGTAGATAGCTCA‑TTGCGGCATAGCGATCCCCGCCTGAAGAGCCTGGGTTGCTACCATATTGACCAGCCAACGTTCCCTTTCGTTACGCTCTTGCTCGATCACTTCCCCGCCTACAGATCGTAGTGCCATCCATTTGGACATCAGAAGCGAAACGAAGGTACCACCAAAACCGAACAGCACGGCCATGatcatc                                                         >  1:88730/1‑218 (MQ=255)                                                                                                                                       cACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAATATTGACCAGCCAACGTTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGAAAc                                                                                                 >  1:71670/1‑144 (MQ=255)                                                                                                                                                      gATCCCCGCATGACGAGCTTGGGTTGCTACAATATTGACCAGCCAACGTTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGAAACGAAGGAACCACCAAAACCGAACAGCAAGGCCATGATCATCAGCCCCTGAACGCTGCTCGACTGTATCCCTGTCAGGCTCAGTACCAGCCCGaaaa  >  1:200578/1‑224 (MQ=255)                                                                                                                                                                      gCCTGGGTTGCTACAATATTGACCAGCCAACGTTCCCTTTCGTTACGCGGTTGATCGATCACTTCCCCGCCAACAGCTCGTAATGCCATCCATTTGAACACCAGAAGCGAAACGAACGAACCACCAACAcc                                                                               >  1:132436/1‑131 (MQ=255)                                                                                                                                                                                     |                                                                                                                                                                                                 TTTCGTGAGCAATTACCGCCTCGGCTTCATCCGGGCTCATGTTCTGCAGCAAACCGGTGCTGACAGCAACCAGAGAGGCATCACGGCGCGCACCGGTTGCAAAAGCGTTGATGTCCGGCGCATGGTAGATAGC‑CACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGTTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGAAACGAAGGAACCACCAAAACCGAACAGCAAGGCCATGATCATCAGCCCCTGAACGCTGCTCGACTGTATCCCTGTCAGGCTCAGTACCAGCCCGAAAA  >  NC_000913/1912156‑1912527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑