Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,099,756	G→A	R325C (CGC→TGC)	ansB ←	L‑asparaginase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,099,756	0	G	A	100.0%	33.9 / NA	14	R325C (CGC→TGC)	ansB	L‑asparaginase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/6);  total (8/6)

ATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGTGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCG  >  NC_000913/3099528‑3099962                                                                                                                                                                                                                                     |                                                                                                                                                                                                               aTAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGTGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCGTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCACGCTTTTTGCGGGTTCAGCGTg                                                                                                                                                                                          <  1:169393/251‑1 (MQ=255)                  aacaaTCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGTGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCACGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGcc                                                                                                                                                                         >  1:85990/1‑251 (MQ=255)                                        cGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGTGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCACGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCg                                                                                                                                                   >  1:196460/1‑251 (MQ=255)                                                       ggAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGAGGTGAGTCTGAAGTGAAACAGCACCGGCCCGATACCGGGGCGAGGCGATTAGTACTGATTGAAGCTCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCACGCTTTTTGAGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGt                                                                                                                                     >  1:122080/1‑250 (MQ=255)                                                                                                                         gAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCACGCTTTTTGCGGGTTCAGCGTGCCNGAGNnnnngann                                                                                                                                                                           >  2:148885/1‑144 (MQ=255)                                                                                                                               nccccGGCACGATACCGGGGCGAGGCGATTAGTACTGTTTGAAGATCTGCTGGATCTGCTGAGGCTCTTTGGTTTGCGTCAGAGCCAGTTGAAACCAAACGAACGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGCAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTAGGTACGCGGGAAGAAAGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGaa                                                             >  1:31819/2‑250 (MQ=255)                                                                                                                                                                      tGAAGATCTGCTGGATCTGCTGCGGTTCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCACGCGTTTTGCGGGGTCAGCTTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGcc                                                                                                                               <  1:140221/145‑1 (MQ=255)                                                                                                                                                                          gatctgctggatctgctgCGGAGCGTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCACGCTGTTTGCGGGTTCAGCGGGCCAGAGGCGACGAAGCCGTAGTTCGGATCATCCACTTCGGCATCCGGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGc                                                                                                         <  1:44177/163‑1 (MQ=255)                                                                                                                                                                                  ngatcngctgCGGATCTTTGGNTTGCGTCAAAGCCAGTTGCNNCNGAACGNACGCt                                                                                                                                                                                                            <  2:100866/55‑1 (MQ=255)                                                                                                                                                                                  ggatctgctgCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCACGCt                                                                                                                                                                                                            >  1:100866/1‑56 (MQ=255)                                                                                                                                                                                           gcGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCACGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCGCGTCGGCATCCTGAGTGGTAGCGCCCGTCGGGACGCGGGAAGAACGCACGGCTGCAGTACCGGTTTTCGCGGCGATCGCCAGCGTGTCGAACACAGATGTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCg  <  1:175199/249‑1 (MQ=255)                                                                                                                                                                                              gATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCACAACGAACGCTTTTTGAGGGATCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGt                                                                                                                                        >  1:100980/1‑112 (MQ=255)                                                                                                                                                                                                       tttGCNTCNGNGCNNGNTGCANCAGAACGCACGCTTTTTGCGGGTTCAGCGTGCCAGAggn                                                                                                                                                                                  >  2:125687/1‑60 (MQ=255)                                                                                                                                                                                                       ttggCGTCAGAGCCAGTTGCGGCAGAACGCACGCTTTTTGCGGGTTCAGCGTGCCAGAGGc                                                                                                                                                                                  <  1:125687/58‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                               ATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGTGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCG  >  NC_000913/3099528‑3099962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑