Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	740,913	G→A	R469R (CGG→CGA)	phr →	deoxyribodipyrimidine photolyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	740,913	0	G	A	100.0%	15.1 / NA	9	R469R (CGG→CGA)	phr	deoxyribodipyrimidine photolyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/3);  total (6/3)

GGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAGCACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTC  >  NC_000913/740674‑741111                                                                                                                                                                                                                                                |                                                                                                                                                                                                       ggAACCGATGCAGCGCCGTATTTTCGTATTTTCAAACCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAGCACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGAAAGGGGAAAt                                                                                                                                                                                              >  1:39644/1‑250 (MQ=255)                                                 ggCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAGCACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGAAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACg                                                                                                                                             >  1:81662/1‑251 (MQ=255)                                                  gCGAGNNATNNNATNNTGAGGGCGAGTTTATCCGCCAGTNGCTACCGGAACTGCGCGATGTGCCAGGGANANTNGCGCNNGAGNCGTGGAAGTGNGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAGNANAAAGAAGCGAGAGTACAAACGTTGGCAGCGTNTGAGGCGGCGCGAAAGGGGAAATANAAGANNCGNcn                                                                                                                                                                                 >  2:184416/1‑213 (MQ=255)                                                                                                                                    gCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAGCACAAAGCAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGAAAGGGGAACTAACAGCCGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTCCAAATTTGTGCAAATTCAAt                                                                                                           >  1:250814/1‑202 (MQ=255)                                                                                                                                                    cAGAAAGCAGGCGTGACGCTGGATTATCGGCAACCGCTAGTCGAGCACAAAGAAGCGAGAGTACAAACGATGGCAGCATATGAGGCGACGCGAAAGGGGAAATAACAGACGCGTCAGGCCATCGAGCCCAGATGCCCGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGGGCAAATTCAAAAAATTGCAGGAAATACGTAAGCCTGATAAGACGCTTCAAGCgctgcagcacgcatagttgcttgc                                          >  1:79180/1‑228 (MQ=255)                                                                                                                                                                                cGCAACCGATAGTCGAGCACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGAAAGTGGAAATAACAGACGCGGCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCATTAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGGCGCATCAGGCATAGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCgg              <  1:184416/251‑1 (MQ=255)                                                                                                                                                                                           gTCGAGCACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGAAAGGGGAAATACCAGAAGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGAGAACGCCTTATCCAGACTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGGTTCTGAAttt   >  1:96602/1‑251 (MQ=255)                                                                                                                                                                                             cGAGCACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGAAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAAtttn  <  1:25568/250‑2 (MQ=255)                                                                                                                                                                                                                            gCAGCGTATTAGGCGGCGAGAAAGGGTAAATAACAGACGCGGCAGGCAATCGAGCCCAGATTCTGGATGCGGCGTGAACGCCCTAGCCAGCCTACAAATATGTTCTAATTCAAGAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAgg                                                       <  1:42597/166‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                       GGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAGCACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTC  >  NC_000913/740674‑741111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑