Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,744,751 Δ1 bp coding (1295/1374 nt) mdtK → multidrug efflux pump MdtK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,744,7510C.90.0% 24.7 / ‑1.5 10coding (1295/1374 nt)mdtKmultidrug efflux pump MdtK
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (5/4);  total (5/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

CATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGTAACTTCCCCTTTCAC  >  NC_000913/1744522‑1744996
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
cATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATAAGCGTTCAATTTTCTATATTACCTTTAAGGCTTACTGGGTGCCGGGCTTGCCCAGGGGCTCTATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGAAGTCGG‑AGCCATTATGCTGATGTTGCGt                                                                                                                                                                                                                                 >  1:136267/1‑251 (MQ=255)
                                  cTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGTCTTACTGGGTGCGGGGCTTGCCAAGCGGCTATATGCTGGCACTGACCGATCTGGTCGTTGAACCTATCTGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGc                                                                                                                                                                                                                                                    <  1:183706/199‑1 (MQ=255)
                                        cAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTAGATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGc                                                                                                                                                                                         <  1:220609/251‑1 (MQ=255)
                                                                                                              tACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGCCAGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGc                                                                                                                                                                                                                             >  1:98815/1‑145 (MQ=255)
                                                                                                                             gCTGGGCTTGCCACGCGGCTATATTCTGGCACTGACCCATCTGGTCGTTGAACCTATGGGGACCGCCGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCCACGAGCATCCCGCTAATAAAGACAAGGCACACCCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATc                                                                                                    >  1:191172/1‑251 (MQ=255)
                                                                                                                                    ttGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGTCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTcn                                                                                              <  1:154572/250‑2 (MQ=255)
                                                                                                                                        cAAGCGGCTATATTCTGGCACTGACCGAGCTGGTCGTTGAACCTATGGGGCCAGCAGGCTGCTGTATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTc                                                                                                                                                                                                                        <  1:109072/124‑1 (MQ=255)
                                                                                                                                                                          cGTTGATCCTATGGGGCCATGAGTCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACTTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGTTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATcc                                                                                                   <  1:89557/207‑1 (MQ=255)
                                                                                                                                                                                                       ggATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCCGCAACGAGCATCCCGCTAATAAAGACAAGGCACAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGtat                          >  1:207022/1‑251 (MQ=255)
                                                                                                                                                                                                                               cGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCCATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGTAACTTCCCCTTTcac  >  1:171458/1‑251 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
CATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGTAACTTCCCCTTTCAC  >  NC_000913/1744522‑1744996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑