Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,955,071	C→T	E311E (GAG→GAA)	recB ←	exodeoxyribonuclease V subunit RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,955,071	0	C	T	100.0%	19.5 / NA	8	E311E (GAG→GAA)	recB	exodeoxyribonuclease V subunit RecB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/1);  total (7/1)

GCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCC‑AATTCGCCACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTT  >  NC_000913/2954892‑2955308                                                                                                                                                                                     |                                                                                                                                                                                                                                              gCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGACATCAACCCCC‑CATCCGCCACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATACGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCTTCAAACAGTGGATGTCGCGGGGTTTccccacggcc                                                                                                                                                                                                            >  1:130621/1‑209 (MQ=255)                                nggAATCGAGCCGACTTAACATGTCATCAAAACCC‑AATTCGCCACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCTTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCAAGCGACTCCGGCAACTGATAACTGttt                                                                                                                                         >  1:67972/2‑251 (MQ=255)                                         gCCGACTTAACATGTCATCAAAACCCCAATTCGCCACGTCGGCGTTAGTCACGCGCTACTGTTTCGCGGATTTCAGCCAGTGCTCGGGTGATCACTAGATCGCGGATCGACAATTGTTTTGCAAGCATTTGATCGATCGCTTCAAACAGTGGATGTCGCGGGGTTTc                                                                                                                                                                                                                     <  1:166844/167‑1 (MQ=255)                                                                                  gcgTTTTTCACGCGCTACTGTTTCGCGGATCTCCGCCAATGCGCGGGTGATCACCAGATCGCGGAACGACAATGGTTCTGCAAGCAGTTGATCGACCGCTTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCCACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGCTCCATTTAGcc                                                                                            >  1:82331/1‑247 (MQ=255)                                                                                                                             cgGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCTTCAAACAGTGGATGTAGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGAt                                             >  1:172169/1‑251 (MQ=255)                                                                                                                                                 gATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCTTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCAccc                          >  1:214767/1‑250 (MQ=255)                                                                                                                                                     gACAATGGTTCTGCAAGCAGTTGATCGATCGCTTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGc                     >  1:69853/1‑251 (MQ=255)                                                                                                                                                                        gTTGATAGATCGCTTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGCCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATAACAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGtttt  >  1:212782/1‑251 (MQ=255)                                                                                                                                                                                     |                                                                                                                                                                                                                                              GCCAACACCTCACCGCTTTCGCTACGCAGCGCGGAATCGAGCCGACTTAACATGTCATCAAAACCC‑AATTCGCCACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTT  >  NC_000913/2954892‑2955308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑