Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,569,129	C→T	intergenic (‑402/+1033)	ytiE ← / ← yjiR	protein YtiE/fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,569,129	0	C	T	100.0%	31.1 / NA	13	intergenic (‑402/+1033)	ytiE/yjiR	protein YtiE/fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/4);  total (9/4)

GCCTGGAAAGCGCCTCGGGGAATGGGAAATTGCCGGGTGAGAATGGTTTTGTTAGTCGCTAAAGTCAGGCCATCTTTTTCAACAGGTGATGGATCGCCATGACAAACTTCACGACCAGCACGCCGCATGATGCATTATTTAAAACCTTTCTCACGCACCCTGACACTGCGCGGGATTTTATGGAGATTCACTTACCTAAAGATTTACGTGAACTGTGCGATCTCGATAGCTTAAAACTGGAATCCGCCAGCTTTGTCGATGAAAAATTGCGGGCGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAGCGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGT  >  NC_000913/4568900‑4569350                                                                                                                                                                                                                                      |                                                                                                                                                                                                                              nccTGGAAAGCGCCTCGGGGAATGGGAAATTGCCGGGTGAGAATGGTTTTGTTAGTCGCTAAAGTCAGGCCATCTTTTTCAACAGGTGATGGATCGCCATGACAAACTTCACGACCAGCACGCCGCATGATGCATTATTTAAAACCTTTCTCACGCACCCTGACACTGCGCGGGATTTTATGGAGATTCACTTACCTAAAGATTTACGTGAACTGTGCGATCTCGATAGTTTAAAACTGGAATCCGCCAGc                                                                                                                                                                                                          >  1:54572/2‑251 (MQ=255)                                                       ntCGCTAAAGTCAGGCCATCTTTTTCAACAGGTGATGGATCGCCATGACAAACTTCACGACCAGCACGCCGCATGATGCATTATTTAAAACCTTTCTCACGCACCCTGACACTGCGCGGGATTTTATGGAGATTCACTTACCTAAAGATTTACGTGAACTGTGCGATCTCGATAGTTTAAAACTGGAATCCGCCAGCTTTGTCGATGAAAAATTGCGGGCGCt                                                                                                                                                                                >  1:158059/2‑223 (MQ=255)                                                                                            gATCGCCATGACAAACTTCACGACCAGCACGCCGCATGATGCATTATTTAAAACCTTTCTCACGCACCCTGACACTGCGCGGGATTTTATGGAGATTCACTTACCTAAAGATTTACGTGAACTGTGCGATCTCGATAGTTTAAAACTGGAATCCGCCAGCTTTGTCGATGAAAAATTGCGGGCGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTg                                                                                                               >  1:20294/1‑251 (MQ=255)                                                                                                 ccATGACAAACTTCACGACCAGCACGCCGCATGATGCATTATTTAAAACCTTTCTCACGCACCCTGACACTGCGCGGGATTTTATGGAGATTCACTTACCTAAAGATTTACGTGACCTGTGCGATCTCGATAGTTTAAAACTGGAATCCGCCAGCTTTGTCGATGAAAAATTGCGGGCGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATCTCTATGTGGTGATTGAACAt                                                                                                          >  1:184826/1‑251 (MQ=255)                                                                                                 ccATGACAAACTTCACGACCAGCACGCCGCATGATGCATTATTTAAAACCTTTCTCACGCACCCTGACACCGCGCGGGATTTTATGTAGATTCACTTACTTAAAGATTTACGTGCACTGTGCGATCCCGAAGGTTTAAAACTAGAACCCGCCAGCTTTGTCGATGAACACTTGCGGACGCAACACTCCGATCTTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGTTTGAAcct                                                                                                          >  1:184815/1‑249 (MQ=255)                                                                                                     gACAAACTTCACGACCAGCACGCCGCATGATGCATTATTTAAAACCTTTCTCACGCACCCTGACACTGCGCGGGATTTTATGGAGATTCACTTACCTAAAGATTTACGTGAACTGTGCGATCTCGATAGTTTAAAACTGGAATCCGCCAGCTTTGTCGATGAAAAATTGCGGGCGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATc                                                                                                         >  1:91890/1‑248 (MQ=255)                                                                                                                                nnnnnnatnnnnNAAANCCTTTCTCACGCACCCTGACACTGCGCGGGATTTTATGGAGATTCACTTACCTAAAGATTTACGTGAACTGTGCGATCTCGATAGTTTAAAACTGGAATCCGCCAGCTTTGTCGATGAAAAATTGCGGGCGCt                                                                                                                                                                                <  2:158059/144‑1 (MQ=255)                                                                                                                                    ncATTATTTAAAACCTTTCTCACGCACCCTGACACTGCGAGGGATTTTATGGAGATTCACTTACCTAAAGATTTACGAGAACTGTACGATCTCGATAGTTTAAAACTGGAATCCGCCAGCTTTGTCGATGAAAAATTGCGGGCGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGAc                                                                                           >  1:49312/2‑231 (MQ=255)                                                                                                                                     cATTATTTAAAACCTTTCTCACGCACCCTGACACTGCGCGGGATTTTATGGAGATTCACTTCCCTAAAGCTTTACGTGACCTGTGCGATCTCGATAGTTTAAAAATGGAATCCGCCAGCTTTGTCGATGAAAACTTGCGGGCGCTACATTCCGATATTTTATGGTCGGTAAAGACCcgc                                                                                                                                              >  1:246710/1‑179 (MQ=255)                                                                                                                                        tattTAAAACCTTTCTCACGCACCCTGACACTGCGTGGGAGTTTATGGAGATTCACTGACCTAAAGATTAACGTGACCTGTGCGATCTCGATAGTTTAAAACTGGAAGCCGCCAGCTGTGGCGATGAAAAATTGCGGGCGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAgg                                                                                                                                         <  1:95308/181‑1 (MQ=255)                                                                                                                                          tttAAAACCTTTCTCACGCACCCTGACACTGCGCGGGATTTTATGGAGATTCACTTACCTAAAGATTTACGTGAACTGTGCGATCTCTATAGTTTAAAACTGGAATCCGCCAGCTTTg                                                                                                                                                                                                      <  1:74343/118‑1 (MQ=255)                                                                                                                                                                        gcgcGGGATTTTATGGAGATTCACTTACCTAAAGATTTACGTGAACTGTGCGATCTCGATAGTTTAAAACTGGAATCCGCCAGCTTTGTCGATGAAAAATTGCGGGCGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATGTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGGTGCGCTATTCCATGGCGGTGATGTAGCGCCatat                                   <  1:110406/251‑1 (MQ=255)                                                                                                                                                                                                         gATTTACGTGAACTGTGCGATCTCGATAGTTTAAAACTGGAATCCGCCAGCTTTGTCGATGAAAAATTGCGGGCGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAGCGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGt  >  1:89825/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                              GCCTGGAAAGCGCCTCGGGGAATGGGAAATTGCCGGGTGAGAATGGTTTTGTTAGTCGCTAAAGTCAGGCCATCTTTTTCAACAGGTGATGGATCGCCATGACAAACTTCACGACCAGCACGCCGCATGATGCATTATTTAAAACCTTTCTCACGCACCCTGACACTGCGCGGGATTTTATGGAGATTCACTTACCTAAAGATTTACGTGAACTGTGCGATCTCGATAGCTTAAAACTGGAATCCGCCAGCTTTGTCGATGAAAAATTGCGGGCGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAGCGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGT  >  NC_000913/4568900‑4569350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑