Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,796	C→T	L107F (CTC→TTC)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,796	0	C	T	100.0%	22.0 / NA	9	L107F (CTC→TTC)	aceB	malate synthase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

CGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGC  >  NC_000913/4215558‑4216025                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                      cGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGGACGTTGCCTGATTTTATTGCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCTGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGTTAGAGCGCAAGATGGTGATCAACGCGTTCAACGCCAAtg                                                                                                                                                                                                                           <  1:248902/251‑1 (MQ=255)                                aCGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCTCGTAGAGATAACTGTCCCGGTAGAGCGTAAGATGGGGATCAACGCGTTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTc                                                                                                                                                                                            <  1:98013/251‑1 (MQ=255)                                                      aTAAACTTCTGGCAGCGCGAATTCAGCAGCAGAAAGCTATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGTTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACaa                                                                                                                                                                       >  1:83628/1‑250 (MQ=255)                                                                                                             gCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGCAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGTTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACg                                                                                                                                                            >  1:1378/1‑206 (MQ=255)                                                                                                                                                  gCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGTTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGc                                                                                                                            >  1:208066/1‑201 (MQ=255)                                                                                                                                                                           cTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGTTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAgg                                                                                                  <  1:147968/202‑1 (MQ=255)                                                                                                                                                                                     agaagaCCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGTTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCTGTTTTGATTTGTCGGGTACGCGGTCTGc                                       <  1:206212/251‑1 (MQ=255)                                                                                                                                                                                                                           gcAAGATGGTGATCAACGCGTTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGAGGTGAGGc  >  1:1206/1‑250 (MQ=255)                                                                                                                                                                                                                                         aaCGCGTTCAACGCCAATGTGAAAGTCTTTATGGCCGAGTTCGAAGATTCACAGGCACCAGACTGGAACAAAGTGATCGACCGGCAAATTAACCTGCGTGCTGCGGTTAACGGCACAATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGccc                                                                            >  1:123030/1‑162 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                      CGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGC  >  NC_000913/4215558‑4216025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑