Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,397,444	G→A	Q908* (CAA→TAA)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,397,444	0	G	A	100.0%	30.9 / NA	12	Q908* (CAA→TAA)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

ACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGT  >  NC_000913/2397241‑2397650                                                                                                                                                                                                            |                                                                                                                                                                                                               aCATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCACCAGCCAACACGGTTAGGTCCGTAACGGCTCTGGAACAGACCCAGCAGGCGAAGTTCGCCAAAGCTCATGAATGCCCCGCACGTGCCAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGAGCCAGCCAGCACCggcg                                                                                                                                                                 >  1:147725/1‑249 (MQ=255)                           tGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCa                                                                                                                                         >  1:117619/1‑249 (MQ=255)                                                 cacGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGAGATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGAGGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTc                                                                                                                 <  1:230796/251‑1 (MQ=255)                                                       tAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGTACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCg                                                                                                            <  1:147547/250‑1 (MQ=255)                                                                               gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACACCCCCCAGCAGCATCACCACCGCTTTGAGGAAGGTCCGCAGGATCTCAATCAGTTCCGGTGCTATCCACCTCATTATtgtg                                                                                                                                                                                                           >  1:218968/1‑131 (MQ=255)                                                                                          cGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGcc                                                                                                                                                          <  1:61379/169‑1 (MQ=255)                                                                                                          tCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCGCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcctg                                                                                                                               <  1:202240/180‑1 (MQ=255)                                                                                                                  gCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTc                                                                                                                     <  1:18622/182‑1 (MQ=255)                                                                                                                                  ccaccaGAAGGATCACAACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGAcgcg                                                       >  1:87080/1‑228 (MQ=255)                                                                                                                                            gATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCAtn                      <  1:77240/251‑2 (MQ=255)                                                                                                                                                                nggTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGt  >  1:151520/2‑251 (MQ=255)                                                                                                                                                                             ntcAATCAGTTCCGGTGATATCCAACTCATTATTTTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGGGACCGTGtt                                                                               >  1:155537/2‑161 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                               ACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGT  >  NC_000913/2397241‑2397650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑