Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	261,468	C→T	R361C (CGT→TGT)	proB →	glutamate 5‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	261,468	0	C	T	100.0%	24.7 / NA	11	R361C (CGT→TGT)	proB	glutamate 5‑kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/5);  total (6/5)

AGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATTAGCGCAACTCTCCAGCCGCGAAAAAAATCGCGTGCTGGAAAAAATCGCCGATGAACTGGAAGCACAAAGCGAAATCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTT  >  NC_000913/261291‑261676                                                                                                                                                                                  |                                                                                                                                                                                                                 aGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACTGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTg                                                                                                                                                          <  1:93401/234‑1 (MQ=255)                                            gCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCTCTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGGCGTTCACTGTGATGACATGATTTCCCGTTAAGGAGCAGGCTGATGCTGGAAc                                                                                                                                                                      <  1:232336/179‑1 (MQ=255)                                                         ccgggATAGCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATGACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACTGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATTAGCGCAACTCTCCAGCCGCGAAAAAAATCGCGTGCTGGAAAAAATCGc                                                                                 <  1:95035/247‑1 (MQ=255)                                                                   gCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACTGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATTAGCGCAACTCTCCAGCCGCGAAAAAAATCGCGTGCTGGAAAAAATCGCCGATGAACTg                                                                       >  1:72583/1‑251 (MQ=255)                                                                            ntCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCAGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACTGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATTAGCGCAACTCTCCAGCCGCGAAAAAAATCGCGTGCTGGAAAAAATAGCCGATGAACTGGAAGcaca                                                               >  1:49676/2‑250 (MQ=255)                                                                                gTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACCCCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACTGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATGGCCGCGAAGCAAGCCTCGTATAAATTAGCGCAACTCTCCAGCCGCGAAAAAAATCGCGTGCTgg                                                                                           <  1:114013/218‑1 (MQ=255)                                                                                                     tACGCCGTATTGCCGGACACCACTCGCAAGAACTTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACTGTGATGACATGCTTCCCCCTTAAGGAGCAGGCTGATGCTCGAACAACTGGGCATTGCCGCGAAGCAAGACTCGTATAAATTACAGCAACTCTCCAGCAGCGAAAAACATCGCGTGCTGGAAAAAATCGCCGATGAACTGGAAGCACaaa                                                             >  1:113078/1‑227 (MQ=255)                                                                                                                                    aaTTGATGCCATACTGGGATATGAATACGGCCCGGTTGCCGTTCACTGTGATGACATGATTACCGGTTAAGTAGCAGGCTGATGCTGGNNCAAATgn                                                                                                                                                                >  2:9085/1‑96 (MQ=255)                                                                                                                                    aaTTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACTGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGNNCAAATGNNc                                                                                                                                                              >  2:16797/1‑99 (MQ=255)                                                                                                                                    aaTTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACTGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATTAgcg                                                                                                                             <  1:16797/132‑1 (MQ=255)                                                                                                                                        gATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACTGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATTAGAGCAACTCTCCAGCCGCGAAAAAAATCGCGTGCTGGAAAAAATCGCCGATGAACTGGAAGCACAAAGCGAAATCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCtt  >  1:188084/1‑251 (MQ=255)                                                                                                                                                                                  |                                                                                                                                                                                                                 AGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATTAGCGCAACTCTCCAGCCGCGAAAAAAATCGCGTGCTGGAAAAAATCGCCGATGAACTGGAAGCACAAAGCGAAATCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTT  >  NC_000913/261291‑261676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑