Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	855,252	C→T	G165S (GGC→AGC)	ldtB ←	L,D‑transpeptidase LdtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	855,252	0	C	T	100.0%	20.6 / NA	9	G165S (GGC→AGC)	ldtB	L,D‑transpeptidase LdtB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/5);  total (4/5)

CTTCGTTACGCAGACGCACACAACCATGACTTACACGCAGGCCGATACCGAAGTTGGCGTTGGTGCCATGGATAGCATACAGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGT  >  NC_000913/855096‑855488                                                                                                                                                             |                                                                                                                                                                                                                                             cTTCGTTACGCAGACGCACACAACCATGACTTACACGCAGGCCGATACCGAAGTTGGCGTTGGTGCCATGGATAGCATACAGGCGACCGATATAGAGTGCATACAGCCCCAGCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCTCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAg                                                                                                                                                                                                   <  1:132043/200‑1 (MQ=255)                 cacacaACCATGACTTACACGCAGGCCGATACCGAAGTTGGCGTTGGTGCCATGGATAGCATACAGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCTCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCCGTTGATAGGCGTATCTTTGCCTAACTGAc                                                                                                                                >  1:116227/1‑251 (MQ=255)                                        ggCCGATACCGAAGTTGGCGTTGGTGCCATGGATAGCATACAGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCTCGCAGCGCGGTACTCTGCGTg                                                                                                                                                                                                                         <  1:202711/139‑1 (MQ=255)                                                         gCGTTGGTGCCATGGATAGCCTACAGGCGACCGATATAGAGTGCATCCAGAACCATCGGATTATCCCGAACTGCCGGAACGACAGACGGCAGCGGTTCGCTAGCAACGCCGTACTCTGCGTGCATTTTGACGGTCGGCGTCCa                                                                                                                                                                                                    >  1:222529/1‑143 (MQ=255)                                                           nttGGTGCCATGGATAGCATACAGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCTCGCAGCGCGGTACTCTGCGTGCATTTTGGc                                                                                                                                                                                                                >  1:50673/2‑129 (MQ=255)                                                                                                     aTACAGCCCCATCGGGTGATCCGGACCGGCCGGAACGACAGCCGGAAGCGGTGCGTTCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCTGCGTCCAGGTCGTTCCTGCTTTTTGACGCTCATCTGTGGTGGTGCAGTTGATAGGCGTATCTTGGTCTAACTGACCAATGTCGATCGTAAGCGCGATAACGTTGTTGGTCCCTTTCGGATAGTAATAAAGACGTATCTCAGCACTGTTAATGTCGATGc                                            <  1:186972/251‑1 (MQ=255)                                                                                                                          cGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCTCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGTGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTAGCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACTGTTGTGGTCCCTTTCGGATAGTAATAAAGACGTATCTCAGc                                                             <  1:222893/213‑1 (MQ=255)                                                                                                                                               cGGAAGCGGTTCGCTCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGTATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGt  >  1:8852/1‑251 (MQ=255)                                                                                                                                                    gCGGTTCGCTCGCAGCGCGGTACTCTGCGTGCATGTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGGTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGgtgct                                                                                                 <  1:239723/151‑3 (MQ=255)                                                                                                                                                             |                                                                                                                                                                                                                                             CTTCGTTACGCAGACGCACACAACCATGACTTACACGCAGGCCGATACCGAAGTTGGCGTTGGTGCCATGGATAGCATACAGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGT  >  NC_000913/855096‑855488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑