Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	820,340	C→T	L153L (CTG→TTG)	ybhL →	Bax1‑I family protein YbhL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	820,340	0	C	T	100.0%	26.3 / NA	11	L153L (CTG→TTG)	ybhL	Bax1‑I family protein YbhL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/7);  total (4/7)

GTAACGACGATGCTCTTTATGCTTTATTCGGCGCTGACGGGTCTTACGCTTTCCAGTATATTCATTGTCTATACCGCTGCTTCTATCGCCAGTACTTTCGTCGTTACTGCCGGGATGTTCGGCGCAATGAGCCTGTACGGTTACACCACGAAGCGCGATTTAAGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGCATTGTGCTGGCATCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTG  >  NC_000913/820133‑820528                                                                                                                                                                                                                |                                                                                                                                                                                             ntAACGACGATGCTCTTTATGCTTTATTCGGCGCTGACGGGTCTTACGCTTTCCAGTATATTCATTGTCTATACCGCTGCTTCTATCGCCAGTACTTTCGTCGTTACTGCCGGGATGTTCGGCGCAATGAGCCTGTACGGTTACACCACGAAGCGCGATTTAAGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGCATTGTGTTGGCATCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGAt                                                                                                                                                   >  1:155063/2‑251 (MQ=255)                                       gggTCTTACGCTTTCCAGTATATTCATTGTCTATACCGCTGCTTCTATCGCCAGTACTTTCGTCGTTACTGCCGGGATGTTCGGCGCAATGAGCCTGTACGGTTACACCACGAAGCGCGATTTAAGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGCATTTTTTGGGCATCGCTGGTCAACGGCTGTTTGAAAAGCGAAGCATTGATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCg                                                                                                             <  1:178432/251‑1 (MQ=255)                                                       aGTATATTCATTGTCTATACCGCTGCTTCTATCGCCAGTACTTTCGTCGTTACTGCCGGGATGTTCGGCGCAATGAGCCTGTACGGTTACACCACGAAGCGCAATTTAAGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGCATTGTGTTGGCATCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGa                                                                                             <  1:247746/251‑1 (MQ=255)                                                                                    tATCGCCAGTACTTTCGTCGTTACTGCCGGGATGTGCGGCGCAATGAGCCTGTACGGTTACACCACGAAGCGCGATTTAAGTGGCTTTGGCAATATGCTGTTGATGGCGGTAATCGGCATTGTGTTGGCAGCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAg                                                                 <  1:35265/250‑1 (MQ=255)                                                                                                       nttACTGCCGGGATGTTCGGCGCAATGAGCCTGTACGGTTACACCACGAAGCGCGTTTTAAGTGGCATTGGCAATATGCTGTTTATGGCGTTTATCGGCATTGTGTTGGCATCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGATGTGGGCAGTTTCCTACATCGGCGTGATTGTCTTTGTTGGATTg                                                                                                        >  1:47738/2‑192 (MQ=255)                                                                                                               cGGGATGTTCGTCGCAATGAGCCTGTACGGTTACACCACGAAGCGCGATTTAAGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGCATTGTGTTGGCATCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCGTATTGACGTCGTATGACACGTAGAAACTGAAAAAGATGGGTGAGCAGATTGATACCCGCGACACGTCGAAcc                                     <  1:168053/251‑1 (MQ=255)                                                                                                                        cGGCGCAATGAGCCTGTACGGTTACACCACGAAGCGCGATTTAAGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGCATTGTGTTGGCATCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGATGTGGGCAGTTACCTACATCGGCGTGATTGTCCTTGTCGGATTGACGGCGTATGACACGCCGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCaaa                             >  1:72704/1‑250 (MQ=255)                                                                                                                                 gAGCCTGAACGGTTACACCACGAAGCGCGATTTTAGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGCATTGTGTGGGCATCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGATGTGGGCATTTACCTACATCGGCGTGAGTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTTAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCAtt                    <  1:133527/250‑1 (MQ=255)                                                                                                                                                         ncgcgATTTAAGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGCCTTGTGTTGGCATCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGATGTGGGCAGTTACCTACATCGGCGTGATTGTATTTGTCGGATTGACGGGGTATGACACGAAGAAACTGAAAACTATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTg  >  1:54777/2‑244 (MQ=255)                                                                                                                                                            ctattgaaGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGGATTGTTTTGGCAGGGCTGGTCAACTTCGGGTTGAAAATCGAGGCATTTATGGGGGAGGTTACCTACAGCGGCGTGATTGGGTTGGTCGGATTTTCTGCGTAGGACACGCAGAAGCTGAAAAATATGGGTGAGCAGATTGATACCCGCGac                                               <  1:238201/190‑1 (MQ=255)                                                                                                                                                                   aGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGCATTGTGTTGGCATCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGgcgc            <  1:210298/224‑1 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                                                                             GTAACGACGATGCTCTTTATGCTTTATTCGGCGCTGACGGGTCTTACGCTTTCCAGTATATTCATTGTCTATACCGCTGCTTCTATCGCCAGTACTTTCGTCGTTACTGCCGGGATGTTCGGCGCAATGAGCCTGTACGGTTACACCACGAAGCGCGATTTAAGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGCATTGTGCTGGCATCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTG  >  NC_000913/820133‑820528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑