Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,865,376	C→T	F92F (TTC→TTT)	ygbN →	putative transporter YgbN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,865,376	0	C	T	100.0%	25.9 / NA	12	F92F (TTC→TTT)	ygbN	putative transporter YgbN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/10);  total (2/10)

AAAGGTACAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGCGGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTAATTATTTCAGTCGCAAGTTAGGTGACAAACGAACTATCGCTGCGCTGACTCTGGCAGCGTTCTTCCTCGGTATTCCCGTCTTCTTTGATGTCGGCTTTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGC  >  NC_000913/2865172‑2865543                                                                                                                                                                                                             |                                                                                                                                                                        aaaGGTACAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGCGGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTAATTATTTTAGTCGCAAGTTAGGTGACAAACGAACTATcgctgcgcn                                                                                                                                   <  1:24085/243‑2 (MQ=255)                            cctcGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGCGGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTAATTATTTTAGTCGcn                                                                                                                                                                  <  1:159305/185‑2 (MQ=255)                              tcGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGCGGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTTGTTCCGTCACTATTATTATTGGTCTGGGCGCTATGCTGTGGCGTATGATCGAACACTCTGGTGGTGGAGAGTCACTGGCTAATTATTTTAGTCGCAAGTTAGGtn                                                                                                                                                         <  1:47878/192‑2 (MQ=255)                                        gTTAGTCGCACTTGCGGCAGGTATACCGGCGGGCCAAGGGGGTAAAGTGGTTATTCCCGGGGTGGGGGGCGTGGTTTTTTCCCTCCCTATTATTCTTCATCTTGGCGCTTCGCTGGGGCGTATGATCCAACACTCTGGTGGTGACGAGGCACTGGCTCATTATTTTAGTCGCACGTTAGGGGACAAACCAACTATCGCTGCGCTGACTCTGGCAGCGGTCTTCCTCGGGATTCCCGTCTTCttttatttcg                                                                                    >  1:99413/1‑244 (MQ=255)                                                      tggCAGGTATACCGGCGGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTAATTATTTTAGTCGCAAGTTAGGTGACAAACGAACTATCGCTGCGCTGACTCTGGCAGCGTTCTTcc                                                                                                               <  1:227056/209‑1 (MQ=255)                                                                 ccGGCGGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTTGTTCCGGCACTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTANTTANNNNNGtnnnnnn                                                                                                                                                                >  2:155479/1‑144 (MQ=255)                                                                    ncgggcgAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTAATTATTTTAGTCGCAAGTTAGGTGACAAACGAACTATCGCTGCGCTGACTCTGGCAGCGTTCTTCCTCGGTATTCCCGtc                                                                                                 >  1:77709/2‑210 (MQ=255)                                                                                 gTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTAATTAGTTTAGTCGCAAGTTAGGTg                                                                                                                                                         <  1:87646/141‑1 (MQ=255)                                                                                      gTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTAATTATTTTAGTCGCAAGTTAGGTGACAAACGAACTAGCGCTGCGCTGACTCTGGCAGCGTTCTTCCTCGGTATTCCCGTCTTCTTTGATGTCGGCTTTATTATTCTTGCGCCAATCATTTACGGGTTTGCCAAGGTTGc                                      <  1:212677/251‑1 (MQ=255)                                                                                                                          gTCACTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTAATTATTTTAGTCGCAAGTTAGGt                                                                                                                                                          <  2:155900/99‑1 (MQ=255)                                                                                                                          gTCACTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTAATTATTTTAGTCGCAAGTTAGGTGACAAACGAACTATCGCTGCGCTGACTCTGGCAGCGTTCTTCCTCGGTATTCCCGTCTTCTTTGATGTCGGCTTTATTATTCTTGCGCCAATCATTTACTGTTTTGCCAATGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGc  <  1:130246/251‑1 (MQ=255)                                                                                                                             aCTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTAATTATTTTAGGCGCAAGTTAGGTGACAAACGAACTATCGCTTCGCGGACTCTGGCAGCGTTCTTCCTCGGTATTCCCGTCTTCTTTGATGTCgg                                                                                   <  1:117807/167‑1 (MQ=255)                                                                                                                                                                                                       ttattTTAGTCGCAAGTTAGGTGACAAACGAACTATCGCTGCGCTGACTCTGGCAGCGTTCTTCCTCGGTATTCCCGTCTTCTTTGATGTCGGCTTTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACGCAAATTTGgcctgn         <  1:67196/167‑2 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                        AAAGGTACAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGCGGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTATTGGTCTGGGCGCTATGCTGGGGCGTATGATCGAACACTCTGGTGGTGCAGAGTCACTGGCTAATTATTTCAGTCGCAAGTTAGGTGACAAACGAACTATCGCTGCGCTGACTCTGGCAGCGTTCTTCCTCGGTATTCCCGTCTTCTTTGATGTCGGCTTTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGC  >  NC_000913/2865172‑2865543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑