Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,914,908	G→A	T209T (ACC→ACT)	proQ ←	RNA chaperone ProQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,914,908	0	G	A	100.0%	30.4 / NA	12	T209T (ACC→ACT)	proQ	RNA chaperone ProQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/7);  total (5/7)

GCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTA‑CAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCG  >  NC_000913/1914699‑1915082                                                                                                                                                                                                                   |                                                                                                                                                                               gcGTCTCTTCCTTTAATACCGGAATTTGCTCAGCACGCGAGCTATCTTCTAACA‑CGAAGGACTGGCCTGCTAATGCAAGCACGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCCAGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGAGGACGCCGCCTTTAGCGATTTCTAATACGGTGGCATCCATCGCATTTTGAccc                                                                                                                                         >  1:167110/1‑249 (MQ=255) gcGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTA‑CAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTGCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTAGTGATTTCTAATACTGTGGCATCCATCGCGTTTTGACCCg                                                                                                                                        <  1:208101/250‑1 (MQ=255)                                                       gCGAAGGTCTGGCTTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTAGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCgggg                                                                                  <  1:234000/251‑1 (MQ=255)                                                         gAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAACAAACATGTTCATGCCTGGCACGGTCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTAGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGgtg                                                                                >  1:39302/1‑251 (MQ=255)                                                           aGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTAGTGATTTCTAATACGGTgg                                                                                                                                                             <  1:6468/172‑1 (MQ=255)                                                                                                     gCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTAGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGGTTTTGGCGCTTtctc                                    <  1:92368/251‑1 (MQ=255)                                                                                                      ccTAAAAAACATGTTCATGCCTGGCCCGGCATCCGTTTCCGAACACCAGGTGTTCTGCGCGGCCAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTAGTGATTTCTCATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCa                                                                                                          >  1:224679/1‑180 (MQ=255)                                                                                                         aaaaaaCATGTTCATGCCTGGCCCTGCCTCCGTTTCAGAACACCAGGTGTTCTGCTCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCCTCTTTAGTGATTTCTAAGACGGTGGCATCCATCGCGTGTTGACCCGCTTTCTCCTTCAGGGCTTGTCCGACAGTCAGAGCTTAAATGTCATAAACCGGTGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTAcc                                <  1:34744/251‑1 (MQ=255)                                                                                                                  gTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTAGTGAATTCGAATACGGGGGCAGCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTgc                       <  1:247819/251‑1 (MQ=255)                                                                                                                     catgcatgGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCAAAACCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTAGTCATTTCTAATAAGGTGGCATCCATCGAGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCAGACAGTCAGAGCTGAAATg                                                                                               >  1:137450/1‑176 (MQ=255)                                                                                                                                    cTCCGNNTCNNNACNNCAGGTGTCCTGCGCGCACAATCANAGACATACT‑GAATTCAGCTGGACGCGGCCNCNGNCTTTNNTGANTTCTACTACGNTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGCNANTCAGAGCTGAAATGTCAGAAACCGGGGTGTGNTGTTCTTCGCGAGGGGCTTTTACTNTTTTNNgcncnnnnnnnnnn                                >  2:185705/1‑213 (MQ=255)                                                                                                                                       cGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTAGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTGTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAgcg  <  1:118215/251‑1 (MQ=255)                                                                                                                                                                                                           ncGTCTTTAGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAAc                                                                                       >  1:29234/2‑98 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                               GCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTA‑CAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCG  >  NC_000913/1914699‑1915082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑