Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,497,794	G→A	intergenic (+1305/‑478)	leuX → / → insC6	tRNA‑Leu/IS2 insertion element repressor InsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,497,794	0	G	A	100.0%	29.8 / NA	11	intergenic (+1305/‑478)	leuX/insC6	tRNA‑Leu/IS2 insertion element repressor InsA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/5);  total (6/5)

AAAGCAGATAAAACAGTTTTATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGCTTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGGAAGAACGGCGATTGATGCTACAGTGGTGGGCCGATTTTCTGGATGTAAACAGAGAAAGGTTTATCAGTCCATTTGAATATGCAAAGATTAATAATCCATTAAAACAGTAATCATCCCGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAA  >  NC_000913/4497556‑4497993                                                                                                                                                                                                                                               |                                                                                                                                                                                                        acaGCAGATAAAACAGTTTTATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCTTGAGTGAGAATACGTTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGATGTTTGTGGTCATGGCTTTCGAACTATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGGCTCTTGAGGCTGTTGAACCTCAGATGAGCAACATGGCGCGAAATTCAATGAGGGCcgcg                                                                                                                                                                                              <  1:34003/248‑1 (MQ=255)                                                     cACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGCCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGCTTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGACATTCAATGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGGAAGAACGGCGATTGATGCTACAgtggtg                                                                                                                                         >  1:179929/1‑251 (MQ=255)                                                                                                                                                        aCAATGGCCTGTAGTTCATTGGTCGANTCAGGTNNGTNCTCTCGTGATGCTGTTGAANGTNNNANNNGNCACATNGANNGAAATTCAANGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGGAAGAACGGCGATTGATGCNACAGTGGTGNNCCGATTTTc                                                                                                                              >  2:61953/1‑163 (MQ=255)                                                                                                                                                        aCAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAATGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGGAAGAACGGCGATTGATGCTACAGTTGTGTGCCGATTTTc                                                                                                                              <  1:61953/163‑1 (MQ=255)                                                                                                                                                                          ttGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAATGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGGAAGAACGGCGATTGATg                                                                                                                                                    <  1:125834/123‑1 (MQ=255)                                                                                                                                                                                          tgtgGTCTCGTGATGCTGTTGAACGTCAGATGAGCCAAATAGCGCGAAATTCCATGAGGGCCGCGTATAACCCTAACGCAGAGCATCTGGAAGAACGGCGATTGATGCCACAGTGGTGGGCCGATTTTCTGGATGTAAACAAAGAAAGGGTTATCAGTCCATTTGAATATGCAAAGATTAATAATCCATTACAACAGTAATCCTCCCGGGCAAATGCCCGGGAATTATTCTAGGATTCTTTTCTTTGTTa     >  1:244760/1‑250 (MQ=255)                                                                                                                                                                                             ggTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAATGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGGAAGAACGGCGCTTGATGCTACCGTGGTGGGCCGATTTTCTGGATGTAAACAAAGAAAGGTTTATCAGTCCATTTGAATATGCCAAGATTAAAAATCCATTAAAACAGTAATCATCCCGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTaaaa  >  1:128674/1‑250 (MQ=255)                                                                                                                                                                                                     gATGCTGGTGAACGTCATATGAGCCACATGGCGCGAAATTCAATGAGGGCCGCGta                                                                                                                                                                                            <  1:122261/56‑1 (MQ=255)                                                                                                                                                                                                     gATGCNGTNGNACNNCNGATGNGCCACATGGCGCGAAATTCAATGAGGGCCGCGta                                                                                                                                                                                            >  2:122261/1‑56 (MQ=255)                                                                                                                                                                                                                                       gAAATTCAATGAGGGCCGCGTAGATCCATAAAGCAGAGCATCTGGAAGAACGGCGATTGATGCTACAGTGGTGGGCCGATTTGCTGGATGTAAACAAATAAAGGTTTATCAGTCCATTTGAATATGCAAAGATTAATAAGCCATTAAAACAGTAATCATCCCGGGCAAATgn                                      <  1:29356/172‑2 (MQ=255)                                                                                                                                                                                                                                          aTTCAATGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGGAAGAACGGCGATTGATGCTACAGTGGTGGGCCGATTTTCTGGATGTAAACAAAGAAAGGTTTATCAGTCCATTTGAATATGCAAAGATTAANAATNNNNNaann                                                             >  2:160737/1‑144 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                        AAAGCAGATAAAACAGTTTTATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGAGTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGCTTTCGAACAATGGCCTGTAGTTCATTGGTCGAATCAGGTCTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGCGCGAAATTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGGAAGAACGGCGATTGATGCTACAGTGGTGGGCCGATTTTCTGGATGTAAACAGAGAAAGGTTTATCAGTCCATTTGAATATGCAAAGATTAATAATCCATTAAAACAGTAATCATCCCGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAA  >  NC_000913/4497556‑4497993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑