Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	631,633	G→A	W580* (TGG→TGA)	cstA →	pyruvate transporter CstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	631,633	0	G	A	100.0%	23.9 / NA	12	W580* (TGG→TGA)	cstA	pyruvate transporter CstA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/8);  total (4/8)

GGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCA  >  NC_000913/631453‑631803                                                                                                                                                                                     |                                                                                                                                                                           ggCAACAGCGCTGTGAGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGTTACCAACGGCCTGGCTGATGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAg                                                                                                        <  1:35430/249‑1 (MQ=255) ggCAACAGCGCTGGGCGTACTGGCGTTGGGCTACTTCCTCCATCAGGGTGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACGAGATGCTGGCAGGGATGTCGCTGATGGTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACTTTACGGTTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGTGTTTAg                                                                                                        <  1:35437/249‑1 (MQ=255)                 gTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGAGGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCTCTGGTACCAACTGCCTGGCTTCTGATTTGTACCCTGAGCGGAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCgg                                                                                      <  1:42889/251‑1 (MQ=255)                                                  gTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGTTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGgctgc                                                                                                                                                <  1:36545/160‑1 (MQ=255)                                                                            cacTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCAGTGGTGTTGTTCAAGATGCAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCa                                                       >  1:217705/1‑223 (MQ=255)                                                                                                     gCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCNACGNTACGNCTNAGTCGCGCTTGTACCNACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAANNCNNNTNGCCCGGAt                                                                                                 <  2:176005/156‑1 (MQ=255)                                                                                                     gCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCCACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGATGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGAt                                                                                                 >  1:176005/1‑156 (MQ=255)                                                                                                                 cTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCCACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCa  >  1:36297/1‑239 (MQ=255)                                                                                                                  tGGCAGGGATGTCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACACCGGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGCCTTCCCGGTCATTGCTAATGAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTc                             <  1:247809/211‑1 (MQ=255)                                                                                                                                         tgtgCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTg                                                                                                                       <  1:163533/98‑1 (MQ=255)                                                                                                                                                                            ttACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGt                                                                                                                                        <  1:3409/46‑1 (MQ=255)                                                                                                                                                                            ttACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGt                                                                                                                                        >  2:3409/1‑46 (MQ=255)                                                                                                                                                                                     |                                                                                                                                                                           GGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCA  >  NC_000913/631453‑631803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑