Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,866,159	C→T	R105H (CGT→CAT)	mipA ←	MltA‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,866,159	0	C	T	100.0%	12.3 / NA	6	R105H (CGT→CAT)	mipA	MltA‑interacting protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/2);  total (4/2)

CCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTAC  >  NC_000913/1865991‑1866322                                                                                                                                                                         |                                                                                                                                                                    ccAATACCCGGAGGCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTATGGTCATCCAGGTGGCGCATTTGGTGATCGCCACTGTCTTTCGCTGTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAg                                                                                   <  1:233928/251‑1 (MQ=255)                  ggTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATCTACCAGACGAAGCACTTGCTGTTATCAAGGGTATAGCCCGCCAGGGTCGCCCGCAGGTAACCGTACTGGGTAACGTGCGCATAAGACAGACCAGCCATCATGGTGCTCTTATGGTCATCCAGGTGAAGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCCGTAAGCGGTAATTGAAAGtt                                                                                 >  1:225335/1‑236 (MQ=255)                                                                                                                                      gAGCATAAGACAGACCAGCCATCATGGTGCTCTTATGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTg                                                                        >  1:226965/1‑129 (MQ=255)                                                                                                                                                      aGCCATCATGGTGCGCTTATGGTCATCCAGGTGACTCATTGGGTGATCTCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGGTGCGTCATTCCACAGGGAGTAACCACCACCTAAGCCACGGAACCAGAAGGTATCGCCTTCATAGTTGATTAc  <  1:176757/183‑1 (MQ=255)                                                                                                                                                      aGCCATCATGGNGCTCTTATGGTCATCCAGGTGACg                                                                                                                                                     >  2:34738/1‑36 (MQ=38)                                                                                                                                                      aGCCATCANGNNNCNNTTATGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTNGCTTTGACGTAAAGCNGCNGCCANTAANCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTAc  >  2:176757/1‑183 (MQ=255)                                                                                                                                                                         |                                                                                                                                                                    CCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTAC  >  NC_000913/1865991‑1866322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑