Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	845,100	C→T	K127K (AAG→AAA)	ybiO ←	moderate conductance mechanosensitive channel YbiO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	845,100	0	C	T	100.0%	27.1 / NA	11	K127K (AAG→AAA)	ybiO	moderate conductance mechanosensitive channel YbiO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

GATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGC  >  NC_000913/844902‑845339                                                                                                                                                                                                       |                                                                                                                                                                                                                                                gATAATAAACGCCCAGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGTTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAGCGGGCGGaa                                                                                                                                                                                              >  1:200429/1‑250 (MQ=255)                          gCCGGAAGCGGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATCCCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGTTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAGCGGGCGTAAAggn                                                                                                                                                                                          <  1:66617/229‑2 (MQ=255)                                                      cACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACAAAAAACTAATACCGCTAACATTGAAAAATGGGTCCGCGGATTGCTGAAGGTTTGTGGATTAAACGGTTTATGCGGGGAGCCGGTGATATTGCGTTAAAGTTGccc                                                                                                                                                                                                           >  1:115276/1‑184 (MQ=255)                                                                     gcgcCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGTTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGtn                                                                                                                         <  1:158129/251‑2 (MQ=255)                                                                                               aGCGGCGGNGNNNANNGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTNACAAATGGGTCAGCGNATNGCTGNAGGNTTGTGGATTAAACGGTTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAgcgg                                                                                                                                                                                                    >  2:176608/1‑150 (MQ=255)                                                                                               aGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATGGAAAAATTGGTCATCGCATTGGTTAAGGTTTGTGGATTATACGGTTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAgcgg                                                                                                                                                                                                    <  1:176608/150‑1 (MQ=255)                                                                                                                        gCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGCTTGTGGATTAAACGGTTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGACCGAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACgggg                                                                      >  1:164258/1‑249 (MQ=255)                                                                                                                                                                   ngTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGTTTATGCGGGGCGCCGGTGATATTGCGATAAAGTTGCCCGAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGAc                                                                                                                                                           >  1:153814/2‑123 (MQ=255)                                                                                                                                                                   ggTCAGCGCATGGCGGAAGGTTTGTGGATTAAACGGTTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGAc                                                                                                                                                           <  2:153814/123‑1 (MQ=255)                                                                                                                                                                                     ggTTTGTGGATTAAACGGTTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAGCGGGCGGAAAGGGCGTCACCATAATGGCGGCTGACTTCGGTGACCt                                                                                                                                                         <  1:105619/107‑1 (MQ=255)                                                                                                                                                                                            ggATTAAACGGTTTAGGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAGCGGGCGGAAAGGGCTTCACCATAAGGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGTCCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGTCGTAGCGGCAACGGTGCCCAACTGTGCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGc  <  1:178848/251‑1 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                                                                GATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGC  >  NC_000913/844902‑845339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑