Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,600,930	G→A	P16S (CCT→TCT)	dnaC ←	DNA replication protein DnaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,600,930	0	G	A	100.0%	28.6 / NA	13	P16S (CCT→TCT)	dnaC	DNA replication protein DnaC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/6);  total (7/6)

TTCATCTGCCCTTCACACTCAACGCGATAGTTCTCAAAGGAACAGTTCTGATGCAGTGGACGAATACCGGAGCGGTTAAAGGTGCGCTGCATTTTCATCGCCCGATTTTCACGTTCGAGAGCGGCGGAGCGGATCGCTCCTTGTTCTTTTTGCCAGGCCAGTAGTTCTTCACCCGTTTTGAATGCGGGTTTGATATGGGCAGGCATCATTTTTTGCAGGCGTTGCATCAGGTCGCCAACGTTTTTCATCGTTACCCTCTGAATCCTGGTGGAATTTGGCTGTCAGGTTCGCTGACCGTATTCACATCTCGTTTCGGCAGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTGCGCGCCAGTTTTTGTTGCCACTGCACATGGTGAAAGACTTTACCTTCCGCCTGCCAGTAGGCAATGAATGAGGCCAGTTCTTCG  >  NC_000913/4600728‑4601164                                                                                                                                                                                                           |                                                                                                                                                                                                                                           ttCATCTGCCCTTCACACTCAACGCGATAGTTCTCAAAGGAACAGTTCTGATGCAGTGGACGAATACCGGAGCGGTTAAAGGTGCGCTGCATTTTCATCGCCCGATTTTCACGTTCGAGAGCGGCGGAGCGGATCGCTCCTTGTTCTTTTTGCCAGGCCAGTAGTTCTTCACCCGTTTTGAATGCGGGTTTGATATGGGCAGACATCATTTTTTg                                                                                                                                                                                                                                <  1:73715/215‑1 (MQ=255)                cacTCAACGCGATAGTTCTCAAAGGAACAGTTCTGATGCAGTGGACGAATACCGGAGCGGTTAAAGGTGCGCTGCATTTTCATCGCCCGATTTTCACGTTCGAGAGCGGCGGAGCGGATCGCTCCTTGTTCTTTTTGCCAGGCCAGTAGTTCTTCACCCGTTTTGAATGCGGGTTTGATATGGGCAGACATCATTTTTTGCAGGCGTTGCATCAGGTCGCCAACGTTTTTCATCGTTACCCTCTGAATCCt                                                                                                                                                                             >  1:8716/1‑251 (MQ=255)                       cgcgATAGTTCTCAAAGGAACAGTTCTGATGCAGTGGACGAATACCGGAGCGGTTAAAGGTGCGCTGCATTTTCATCGCCCGATTTTCACGTTCGAGAGCGGCGGAGCGGATCGCTCCTTGTTCTTTTTGCCAGGCCAGTAGTTCTTCACCCGTTTTGAATGCGGGTTTGATATGGGCAGACATCATTTTTTGCAGGCGTTGCATCAGGTCGCCAACGTTTTTCATCGTTACCCTCTGAATCCTGGTGGa                                                                                                                                                                       >  1:188648/1‑250 (MQ=255)                                                                          ggTTAAAGGAGCGCTGCATTTTAATCGCCCGATTTTCACGTTCGAGAGCGCCGGCGCGGATTGCTCCTTGTTCTTTTTTCCAGACCAGTAGATCTTCACACATTTAAAATGCGGGATTGATATGGGAAGACAACATTTTCTGCAGGCGTAGCATCAGGTCGACACCGTTTTTCATAGTTACACTCTGAATCATGGTGGAATTTGGCTATCAGGTTCGCTGACAGCATTCACATATCGTTTCACCAGTccgc                                                                                                                   >  1:191326/1‑251 (MQ=255)                                                                                 ggTGCGCTGCATTTTCATCGCCCGATTTTCACGTTCGAGAGCGGCGGAGCGGATCGCTCCTTGTTCTTTTTGCCAGGCCAGTAGTTCTTCACCCGTTTTGAATGCGGGTTTGATATGGGCAGACATCATTTTTTGCAGGCGTTGCATCAGGTCGCCAACGTTTTTCATCGTTACCCTCTGAATCCTGGTGGAATTTgn                                                                                                                                                                 <  1:49865/198‑2 (MQ=255)                                                                                      gcTGCATTTTCATCGCCCGATTTTCACGTTCGAGAGCGGCGGAGCGGATCGCTCCTTGTTCTTTTTGCCAGGCCAGTAGTTCTTCACCCGTTTTGAATGCGGGTTTGATATGGGCAGACATCATTTTTTGCAGGCGTTGCATCAGGTCGCCAACGATTTTCATCGTTACCCTCTGAATCCTGGTGGAATTTGGCTGTCATGTTCGCTGACCGTATTCACATCTCGTTTCGGCAGTCCGCCGTTGCTGGCAc                                                                                                       <  1:44075/251‑1 (MQ=255)                                                                                                    gCCCGATTTTCACGTTCGAGAGCGGCGGAGCGGATCGCTCCTTGTTCTTTTTGCCAGGCCCGTAGTTATTCACCCGCTTTGAATGCGGGTTTGATATGGGAAGACAACATTTTTTGCAGGCGTTGCAACAGGGCGCCAACGTTTTTCATCGTTACCCTCTGAATCCTGCTGGAATTTGGc                                                                                                                                                                >  1:179551/1‑180 (MQ=255)                                                                                                    gCCCGATTTTCACGTTCGAGAGCGGCGGAGCGGATCGCTCCTTGTTCTTTTTGCCAGGCCAGTAGTTCTTCACCCGTTTTGAATGCGGGTTNAATNTGGANAGNCATCATTTTTTGCAGNCGTTGCATCAGGTCGCCAACGTTTTTCATCGTTACCCTCTGAATNNTNNNGNAATTTGGc                                                                                                                                                                <  2:179551/180‑1 (MQ=255)                                                                                                                    cGAGAGCGGCTGAGCGGATCGCGCCTTGTTCTTTTTGACAGGCCAGTAGTTCTTCACCCGTTTTGAATGCGGGTTTGATATTGGCAGACATCATTTTTTGCAGGCGTTGCATCAGGTCGCCAACGTTTTTCATNGTTNNNnnctnn                                                                                                                                                                                  >  2:153471/1‑144 (MQ=255)                                                                                                                                      tCGCTCCTTGTTCTTTTTGCCAGGCCAGTAGTTCTTCACCCGTTTTGAATGCGGGTTTGATATGGGCAGACATCATTTTTTGCAGGCGTTGCATCAGGTCGCCAACGTTTTTCATCGTTACCCTCTGAATCCTGGTGGAATTTGGCTGTCAGGTTCGCTGAcc                                                                                                                                               <  1:82433/163‑1 (MQ=255)                                                                                                                                                        gccaggccagTAGTTCTTCACCCGTTTTGAATGCGGGTTTGATATGGGCAGACATCATTTTTTGCAGGCGTTGCATCAGGTCGCCAACGTTTTTCATCGTTACCCTCTGAATCCTGGTGGAATTTGGCTGTCAGGTTCGCTGACCGTATTCACAGCTCGTTTCGGCAGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTGCGCGCCAGTTTTTGTTGCCACTGCACATGGTGAAAGACTTTACCTTcc                                      <  1:81217/250‑1 (MQ=255)                                                                                                                                                         ccaggccagTAGTTCTTCTCCCGTTTGGAATGCGGGTTTGATATGGGCAGACATCATTTTTTGCAGGCGTTGCATCAGGTCGCCAACGTTTTTCATCGTTTCCCTCTGAATCCTGGTGGAATTTGGCTGTCAGGTTCGCGGACCGTATTCACGTCTCGTTTCGGCAATCCGCCGTTGCTTGCACTACCTATTTGCATGCTGCGCGCCAGTTTTTGTTGCCACTGCACATGGTGAAAGACTTTACCTTCCGc                                    <  1:180623/251‑1 (MQ=255)                                                                                                                                                                    gtTCTTCACCCGTTTTGAATGCGGGTTTGATATGGGCAGACATCATTTTTTGCAGGCGTAGCATCAGGACGCCAACGTTTTTCATCGTTACCCTCTGCATCCTGGTGGAATTTGGCTGTCAGGTTAGCTGACCGTATTCACATCCCGTGTCGGCAGTCCGCCGTTGCTgg                                                                                                          >  1:252237/1‑170 (MQ=255)                                                                                                                                                                                           ggTTTGATATGGGCAGACATCATTTTTTGCAGGCGTTGCATCAGGTCGCCAACGTTTTTCATCGTTACCCTCTGAATCCTGGTGGAATTTGGCTGTCAGGTTCGCTGACCGTATTCACATCTCGTTTCGGCCGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTGCGCGCCCGTTTTTGTTGCCACTGCACATGGTGAAAGACTTTACCTTCCGCCTGCCAGTAGGCAATGAATGAGGCCAGTTCTTCg  >  1:173944/1‑251 (MQ=255)                                                                                                                                                                                                           |                                                                                                                                                                                                                                           TTCATCTGCCCTTCACACTCAACGCGATAGTTCTCAAAGGAACAGTTCTGATGCAGTGGACGAATACCGGAGCGGTTAAAGGTGCGCTGCATTTTCATCGCCCGATTTTCACGTTCGAGAGCGGCGGAGCGGATCGCTCCTTGTTCTTTTTGCCAGGCCAGTAGTTCTTCACCCGTTTTGAATGCGGGTTTGATATGGGCAGGCATCATTTTTTGCAGGCGTTGCATCAGGTCGCCAACGTTTTTCATCGTTACCCTCTGAATCCTGGTGGAATTTGGCTGTCAGGTTCGCTGACCGTATTCACATCTCGTTTCGGCAGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTGCGCGCCAGTTTTTGTTGCCACTGCACATGGTGAAAGACTTTACCTTCCGCCTGCCAGTAGGCAATGAATGAGGCCAGTTCTTCG  >  NC_000913/4600728‑4601164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑