Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,214,463	C→T	S338N (AGT→AAT)	bluF ←	blue light‑ and temperature‑regulated antirepressor BluF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,214,463	0	C	T	100.0%	30.3 / NA	12	S338N (AGT→AAT)	bluF	blue light‑ and temperature‑regulated antirepressor BluF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

CGATGATTTACTGAAATCATGTGAAAGAATGTGCTGAAAATTATTTTTTCTCTGGCCACGCTATGGAAGGGATACCATTCAATTTAGCTTTAGCAAACAGATCTCCCTGAAACATCTCAATTCCTGCGGATTCAAGCCACATCCACTCTTCTGGTGTTGCCACGCCCATAGCACTGACTTGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCACTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTC  >  NC_000913/1214224‑1214668                                                                                                                                                                                                                                                |                                                                                                                                                                                                              cGATGATTTACTGAAATCATGTGAAAGAATGTGCTGAAAATTATTTTTTCTCTGGCCACGCTATGGAAGGGATCCCATTCAATTTAGCTTTAGCAAACAGATCTCCCTGAAACATCTCAATTCCTGCGGATTCAAGCCACATCCACTCCTCTGGTGTTGCCACGCCCATAGCACTGACTTGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCATTTTTATGAAc                                                                                                                                                                                                     >  1:252055/1‑250 (MQ=255)               aaTCATGTGAAAGAATGTGCTGAAAATTATTTTTTCTCTGGCCACGTTATGGAAGGGATACCATTCAATTTAGCTTTAGCAAACAGATCTCCCTGAAACATCTCAATTCCTGCGGATTCAAGCCACATCCACTCTTCTGGTGTTTCCTCGCCCATATCACTGACTTGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCATTTTTATGAACATTGGTAATCAATTc                                                                                                                                                                                      <  1:180474/251‑1 (MQ=255)                                           attTTTTCTCTGGCCACTCTATGGAAGGGATACCATTCAATTTAGCTTTAGCAAACAGATCTCCCTGAAACATCTCAATTCCTGCGGATTCAAGCCACATCCACTCTTCTGGTGTTGCCTCGCCCATAGCACTGACTTGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCATTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTg                                                                                                                                                           <  1:207643/250‑1 (MQ=255)                                                    ctGGCCACGCTATGGAAGGGATACCATTCAATTTAGCTTTAGCAAACAGATCTACCTGAAACATCTCAATTACTGCGGATTCAAGCCACATCCACTCTTCTGGTGTTGCCACGCCCATAGCACTGACTTGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCATTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGc                                                                                                                                                             >  1:206681/1‑239 (MQ=255)                                                                                       gctttatcAAACATATCTCCCTGAACCATCTCAATTCCTGCGGATTCAAGCCACATCCACTCTTCTGGTGTTGCCACGCCCATAGCACTGACTTGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCATTTTTATGa                                                                                                                                                                                                       <  1:22761/162‑1 (MQ=255)                                                                                                                          tCCTGCGGATTCAAGCCACATCCACTCTTCTGGTGTTGCCACGCCCATAGCACTGACTTGAATTTCAAGTGATGTACAGCATGTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCATGTTGATGAACATTGGTAATCTATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGTAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGc                                                                            <  1:178893/250‑1 (MQ=255)                                                                                                                                                           tgtTGCCTCGCCCATAGCACTGACTTGAATTTCAAGTGATGTACAGCATGTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCATTTTTATGAACATTGGTAATc                                                                                                                                                                                           <  1:123430/106‑1 (MQ=255)                                                                                                                                                               gCCACGCCCATAGCACTGACTTGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGCGGCCCATTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACcag                                                                                       >  1:127247/1‑202 (MQ=255)                                                                                                                                                                       cATAGAACTGACTTGACTTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCATTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTACTTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCAc                              >  1:205154/1‑251 (MQ=255)                                                                                                                                                                                              gTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCATTTTTATGAACAGTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTg                                                                                                                                                           <  1:169521/103‑1 (MQ=255)                                                                                                                                                                                                                       cTGAATAATTGCCTGCCGTGGCCCATTTTTATGAACATTGGTTATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCATCCTTTAGCGATTTAATCGCTTCGGCAAACGCATCAAACCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTc  <  1:35521/231‑1 (MQ=255)                                                                                                                                                                                                                                          ggCCCNTTNNTANNAACNTTGGTAATCAATTCCTGCCTGATTTTAATTCTGTc                                                                                                                                                                 >  2:133586/1‑53 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                              CGATGATTTACTGAAATCATGTGAAAGAATGTGCTGAAAATTATTTTTTCTCTGGCCACGCTATGGAAGGGATACCATTCAATTTAGCTTTAGCAAACAGATCTCCCTGAAACATCTCAATTCCTGCGGATTCAAGCCACATCCACTCTTCTGGTGTTGCCACGCCCATAGCACTGACTTGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCACTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTC  >  NC_000913/1214224‑1214668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑