Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,290,881	C→T	L214F (CTT→TTT)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,290,881	0	C	T	100.0%	13.4 / NA	7	L214F (CTT→TTT)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/1);  total (6/1)

GTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGG  >  NC_000913/1290638‑1291017                                                                                                                                                                                                                                                    |                                                                                                                                         gTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCCGCGGCGAAATTATTCCAGGAACTACAACCGCCGGTTCAGCAGGTGCTTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGCAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTtgctgc                                                                                                                                   >  1:216139/1‑251 (MQ=255)                                                                              ggAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATAGTCACTTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGt                                                         >  1:240783/1‑248 (MQ=255)                                                                                                      ggTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGACGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGttat                                                                                                                           >  1:191553/1‑158 (MQ=255)                                                                                                      ggTGATTTCCCANTNCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTNCTTGATATTGNCGCNNTTTCNGNANACGATCTGGCATTTTATTGCCTTGATGTCNCCCGAGCTGGACATAATGGCGTANNTGNNNCCNNGttat                                                                                                                           <  2:191553/158‑1 (MQ=255)                                                                                                                                                                                 cGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGATGCATTGTTATTACGCGCATTGTTTAACGTATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGCTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCggg  >  1:245299/1‑204 (MQ=255)                                                                                                                                                                                                                                  gCTGGNCATAANGNNGTCTTTGCTGCCTTGTTATNACGCGCATTGTTTAANGGATNATTACNGGna                                                                                           >  2:108260/1‑66 (MQ=255)                                                                                                                                                                                                                                  gCTGGACATAAGGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTGGCACACCAAAAGCAACGGTTGCCAGAGTTGGGCGCGGTATTGAAGCAGGTAAACCATTTACTTCGTc                  <  1:108260/139‑1 (MQ=255)                                                                                                                                                                                                                                        cATAATGGCGTATTTGATGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGtt                                                                 >  1:195421/1‑86 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                         GTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGG  >  NC_000913/1290638‑1291017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑