Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,879,192	G→A	P22S (CCA→TCA)	yeaQ ←	PF04226 family protein YeaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,879,192	0	G	A	100.0%	28.1 / NA	12	P22S (CCA→TCA)	yeaQ	PF04226 family protein YeaQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/5);  total (7/5)

GCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGAAGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCAC  >  NC_000913/1878976‑1879349                                                                                                                                                                                                                         |                                                                                                                                                              gCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGAAGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGACATGATCCACTTCGCCAGAATACCGGCAATAAgn                                                                                                                             <  1:159936/251‑2 (MQ=255)  ccTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCCCAAATAACGGCAACCACGAAGCTGCAAAAATTGAAGCCATCGACTTTCCCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGACATGATCCACTTCGCCAGAATACCGGCAATAAGc                                                                                                                             >  1:179539/1‑250 (MQ=255)           aGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGAAGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGACATGATc                                                                                                                                                        <  1:21876/214‑1 (MQ=255)                                                                                    gCAACCACGAAGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCCGGCTGATCCATGCGCCGACTACGGCACCTACTATCCCCAGCATGATAGTCATAAAGAATCCACCTCCATCTTTACCTGACATGCTCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATGGTTTc                                                                                          <  1:244586/203‑1 (MQ=255)                                                                                                           gAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGACATGATcc                                                                                                                                                       >  1:96971/1‑119 (MQ=255)                                                                                                                    gACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGACATGATCCACt                                                                                                                                                    >  1:19217/1‑113 (MQ=255)                                                                                                                             aaaGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCACAGAAGGATAGTCATAAAGAATCCACCTCAATCTTTACCTGACATGATCCACTTCGACAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGACTGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGTGGGTTAGTCGCTGATAAAAAGCATAGCACAACATAGGGAggcgatcggaagagaacacggctga  >  1:174610/1‑227 (MQ=255)                                                                                                                                gCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGACATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAggg                        >  1:89392/1‑225 (MQ=255)                                                                                                                                            ncTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGACATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGca                                      >  1:78195/2‑199 (MQ=255)                                                                                                                                                        gccgACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGACATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAggn                        <  1:76382/201‑2 (MQ=255)                                                                                                                                                               ncNGCNCCGACNATCCNCAGCAGGATAGTCATNAAGAATCCACCTCCATCTTNNCNTGACANGATcc                                                                                                                                                       <  2:96971/66‑1 (MQ=255)                                                                                                                                                                                                       cACCTCCATCTTTACCTGACATGATTCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTa                                                                    >  1:80128/1‑110 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                              GCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGAAGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCAC  >  NC_000913/1878976‑1879349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑