Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	474,765	C→T	E133K (GAA→AAA)	tesB ←	acyl‑CoA thioesterase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	474,765	0	C	T	100.0%	26.7 / NA	12	E133K (GAA→AAA)	tesB	acyl‑CoA thioesterase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/8);  total (4/8)

GCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTT  >  NC_000913/474601‑474925                                                                                                                                                                     |                                                                                                                                                                 gCTACCATTTGCGCGGATCCACACCGGACGAGGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGTTCGCAGAGGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTTCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGTATTGTTTTTTGATGTGCGAACCCCGCGTCTGGTGCCTGGAAAGATGCAGTCa                                                                            <  1:82353/251‑1 (MQ=255)    aCCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTTCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCatat                                                                         >  1:221269/1‑251 (MQ=255)                       cccTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTTCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTGCGAAACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGtt                                                      <  1:115702/250‑1 (MQ=255)                                         gCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTTCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCcg                                    <  1:22396/251‑1 (MQ=255)                                              gTGACCTTTCAGGGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGAGGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCCCCAGCGATTGGGCGATTTGCGTTGTCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATTTTCGAAACCCGCTTCTGGTGCCGGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCTTTTTGAATAGCAGCAACCCggcggg                               <  1:211211/251‑1 (MQ=255)                                                   cTTTCAGTGGGTTATGAATCTCCGCCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATGGGGCGATTTGCTTTTTCTAAGGGAGGCCAGCAGGCGCTGGCGCGGACGGCATTGTTTTATGATGTTc                                                                                                               <  1:161537/166‑1 (MQ=255)                                                                      cTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGAGATTGGGCGATTTGCGTTTTCGACGGGAGGCCATCCGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCTTCTGGTGc                                                                                             >  1:213703/1‑165 (MQ=255)                                                                           ccGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTTCGAAGGGAGGCCATCAGGCGCTGGCTCGGACGGCATTGTTTTTTGATGTTCGAATCCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCTGTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGtt  <  1:121544/251‑1 (MQ=255)                                                                                                  tCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTTCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGa                                                                                                                    >  1:17961/1‑114 (MQ=255)                                                                                                   cGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTTCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCTTCTGGTGCCTg                                                                                          >  1:246596/1‑139 (MQ=255)                                                                                                    gCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTGTCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGc                                                                                                                                <  1:74240/100‑1 (MQ=255)                                                                                                                    ttCAGCNNTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTTCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGa                                                                                                                    <  2:17961/96‑1 (MQ=255)                                                                                                                                                                     |                                                                                                                                                                 GCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCTTCTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTT  >  NC_000913/474601‑474925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑