Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,980,511	C→T	S42N (AGC→AAC)	ygeA ←	amino acid racemase YgeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,980,511	0	C	T	100.0%	15.7 / NA	8	S42N (AGC→AAC)	ygeA	amino acid racemase YgeA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/2);  total (6/2)

CAAGCGCCGCCTCAGCCAGAATGTCCCCGGTTTTATCCCATTCCCCGCGACGCTGGCACTCTTCTATTTCATGAAAATCGACGCTATGTAGCAGCACTTGCGCAGAGTGAAGCCCACCAAGCCGCTGTTTAATGCCTTCATTTATCAAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGG  >  NC_000913/2980428‑2980678                                                                                    |                                                                                                                                                                        cAAGCGCCGCCTCAGCCAGAATGTCCCCGGTTTTATCCCATTCCCCGCGACGCTGGCACTCTTCTATTTCATGAAAATCGACGTTATGTAGCAGCACTTGCGCAGAGTGAAGCCCACCAAGCCGCTGTTTAATGCCTTCATTTATCAAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAgg  >  1:56016/1‑251 (MQ=255)      gccgccTCAGCCAGAATGTCCCCGGTTTTATCCCATTCCCCGCGACGCTGGCACTCTTCTATTTCATGAAAATCGACGTTATGTAGCAGCACTTGCGCAGAGTGAAGCCCACCAAGCCGCTGTTTAATGCCTTCATTTATCAAAc                                                                                                       >  1:232765/1‑145 (MQ=255)            tCAGCCAGAATGTCCCCGGTTTTATCCCATTCCCCGCGACGCTGGCACTCTTCTATTTCATGAAAATCGACGTTATGTAGCAGCACTTGCGCAGAGTGAAGCCCACCAAGCCGCTGTTTAATGCCTTCATTTATCAAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAGCAACCCAAt                                                      >  1:92846/1‑188 (MQ=255)               nccAGAATGTCCCCGGTTTTATCCCATTCCCCGCGACGCTGGCACTCTTCTATTTCATGAAAATCGACGTTATGTAGCAGCACTTGCGCAGAGTGAAGCCCACCAAGCCGCTGTTTAATGCCTTCATTTATCAAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGAtg                              >  1:66349/2‑209 (MQ=255)                                     cccATTCCCCGCGACGCTGGCACTCTTCTATTTCATGAAAATCGACGTTATGTAGCAGAACTTGCGCAGAGTGAAGCCCACAAAGCCGCTGTTTAATGCCTTCATTTCTCCAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAg                                                               >  1:223599/1‑154 (MQ=255)                                                    gCTGGCACTCTTCTATTTNATGAAAATCGACGTTATGTAGCANNACNNNCGNNGAGTg                                                                                                                                                <  2:189218/58‑1 (MQ=255)                                                    gCTGGCACTCTTCTATTTCATGAAAATCGACGTTATGTAGCAGCACTTGCGCAGAGTg                                                                                                                                                >  1:189218/1‑58 (MQ=255)                                                               tctATTTCATGAAAATCGACGTTATGTAGCAGCACTTGCGCAGAGTGAAGCCCACCAAGCCGCTGTTTAATGCCTTCATTTATCAAACGATATTAAGGAATGGTGGATTCCCAGCGCATTCCTCCCAg                                                               <  1:210685/128‑1 (MQ=255)                                                                                    |                                                                                                                                                                        CAAGCGCCGCCTCAGCCAGAATGTCCCCGGTTTTATCCCATTCCCCGCGACGCTGGCACTCTTCTATTTCATGAAAATCGACGCTATGTAGCAGCACTTGCGCAGAGTGAAGCCCACCAAGCCGCTGTTTAATGCCTTCATTTATCAAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGG  >  NC_000913/2980428‑2980678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑