Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,606,028	G→A	E326E (GAG→GAA)	lsrB →	Autoinducer‑2 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,606,028	0	G	A	100.0%	10.4 / NA	7	E326E (GAG→GAA)	lsrB	Autoinducer‑2 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/2);  total (5/2)

GCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCCCT  >  NC_000913/1605841‑1606168                                                                                                                                                                                            |                                                                                                                                             gCCTGTGGGATGTGGTTCAGCAAGGCCAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAACGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCgg                                                                               >  1:202555/1‑251 (MQ=255)                                                                  ggATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCNGGTNGAAGNCTNGCCAAACAGCGTTCANGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCNNANNNCNTTATATTc                                                                                                                                  <  2:176686/135‑1 (MQ=255)                                                                  ggATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGCAGTCTCGCCAAAAAGCGTTCAGGGCTATGACTACGCAGCGGATGGTAATGGCATCGTACTGTTACCGGAACGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCa               >  1:62691/1‑250 (MQ=255)                                                                  ggATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAACGCGTGATATTc                                                                                                                                  >  1:176686/1‑135 (MQ=255)                                                                           aannNNNGCGNCAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAACGCGTGATATTCAACAAAGAGAATATCGGc                                                                                                                <  2:158835/144‑1 (MQ=255)                                                                              aCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAACGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCCCt  >  1:203476/1‑251 (MQ=255)                                                                                                                        gTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAACGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATccc   >  1:139968/1‑208 (MQ=255)                                                                                                                             gCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAACGCGTGATATTCAACAAAGAGAATATCGGCTAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTc                               <  1:105009/175‑1 (MQ=255)                                                                                                                                                                                            |                                                                                                                                             GCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCCCT  >  NC_000913/1605841‑1606168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑