Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,482,228	G→A	P537L (CCG→CTG)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,482,228	0	G	A	100.0%	32.3 / NA	12	P537L (CCG→CTG)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/4);  total (8/4)

CAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATACCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCG  >  NC_000913/4482004‑4482419                                                                                                                                                                                                                                 |                                                                                                                                                                                                cAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACAGCACCTGCGGtt                                                                                                                                                                                     >  1:35344/1‑237 (MQ=255)             gCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCAAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACAGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGc                                                                                                                                                           >  1:60815/1‑251 (MQ=255)                                     tctcGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACAGCACCTGCGGTTTGCCATTTTCAACTTtgat                                                                                                                                                                  <  1:6009/220‑1 (MQ=255)                                                                 cAGCTGCGGCTGCATCATATTGCCGGTACGTTTTGCCAGCAGTGCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTAGACATCTTCTGGCCTTCGTCATCACGAATCATGCCGGTCATGTAAACGGTGTGGAACAGCACCTGCGGTTTTCCATTTTCATCGTTGATGAATTTCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAg                                                                                                       <  1:245625/251‑1 (MQ=255)                                                                                                             ntGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACAGCACCTgagg                                                                                                                                                                                       >  1:30629/2‑124 (MQ=255)                                                                                                                                                  gATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGGGTGGAACAGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGc                                                                                                                                    >  1:22700/1‑141 (MQ=255)                                                                                                                                                                        gACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACAGCACCTGCGGTTTGCCAttttn                                                                                                                                                                           <  1:157315/80‑2 (MQ=255)                                                                                                                                                                        gACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACAGCACCTGCGGTTTGCCATTTTc                                                                                                                                                                           >  2:157315/1‑80 (MQ=255)                                                                                                                                                                        gACATCTTCTGGCCTTCGTCATCACGAATAAGGCCGGTCATGTAAACGGTGTGGAACAGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATACCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCg  >  1:74219/1‑249 (MQ=255)                                                                                                                                                                                  ngCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACAGCACCTGCGGCTTGc                                                                                                                                                                                  >  1:52497/2‑63 (MQ=255)                                                                                                                                                                                           catcaCGAATCAGGCCGGTCATGTAAACGGTGTGGAACAGCACCTGCGGTTTGCCATTTTCATCTTTGATGACGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATACNCTCNNNNNggnn                                                                                      >  2:149578/1‑144 (MQ=255)                                                                                                                                                                                           catcaCGAATCAGGCCGGTCATGTAAACGGTGTGGAACAGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATACCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTAttttn                                                      <  1:149578/178‑2 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                CAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATACCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCG  >  NC_000913/4482004‑4482419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑