Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,547,902	G→A	G168S (GGT→AGT)	fdnG →	formate dehydrogenase N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,547,902	0	G	A	100.0%	15.3 / NA	7	G168S (GGT→AGT)	fdnG	formate dehydrogenase N subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/5);  total (2/5)

AAGGGGCCGGTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTACCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTCTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGTAGCAAGTCTTGCTCCAACATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTC  >  NC_000913/1547681‑1548117                                                                                                                                                                                                                              |                                                                                                                                                                                                                        aaGGGGGCGGTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTACCCGGAATATCGTGCGCCAGGTTCGGACAAATGGCAGCGCATGAGCTGGGAAGAAGCATTCTCCCGGAGTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTGATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGGTGGCTTTCTACCGGTATGCTGTTTGCCTCCAGTGCCAGCAACGAAACCGGGATGCTGAcn                                                                                                                                                                                            <  1:29487/251‑2 (MQ=255)                                                    cGGAATATCGTGCGCCAGGTTCTGACAAATGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTCTACCGGTATGCTGTGTGCCTCCAGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGgcgcgc                                                                                                                                         <  1:6805/251‑1 (MQ=255)                                                                          tGACAAATGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTCTACCGGTATGCTGTGTGCCTCCAGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAg                                                                                                                                                       <  1:93087/215‑1 (MQ=255)                                                                                                                   gTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTCTACCGGTATGCTGTGTGCCTCCAGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGt                                                                                                                     <  1:18586/208‑1 (MQ=255)                                                                                                                                                              tGAAAAGAAAGAGCAGGGCGTAACGGTAAACAGTTGGCTTTCTACCGGTATGCTGTGTGCCTCCAGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGTAGCAAGTCTTGCTCCAACATTTGGTCGCGGGGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGtgatggtgatgg                               >  1:80770/1‑251 (MQ=255)                                                                                                                                                                                              gTTGGCTTTCTACCGGTATGCTGTGTGCCTCCAGTGCCAGCAACGAAACCGGGCTGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGTAGCAAGTCTTGCTCCAACATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGAGGCAACGCTGCTGAAGCGCATCCCGTc  >  1:18522/1‑248 (MQ=255)                                                                                                                                                                                                  gCTTTCTACCGGTATGCTGTGTGCCTCCAGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGTAGCAAGTCTTGCTCCAACATTTGGTCGCGGTGCGATGa                                                                               <  1:3592/167‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                        AAGGGGCCGGTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTACCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTCTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGTAGCAAGTCTTGCTCCAACATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTC  >  NC_000913/1547681‑1548117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑