Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,760,972	G→A	W142* (TGG→TGA)	yfjJ →	protein YfjJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,760,972	0	G	A	100.0%	26.0 / NA	12	W142* (TGG→TGA)	yfjJ	protein YfjJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/8);  total (4/8)

AGCAAAGCTTAAACATGATAAGCATATTAAAACTCAACGGAAAGACTGGCCTGATAGTCGACATTCCACTTTACGTTACGCATGGGTCAGAGAATATACCAAAAATAGAAAGCGGCATTACCATTTGATACTGTGTTTCAATCAGGATGCTTATTATCATTTAGGTGATTACGACTTAAACCGTAACACGTTACGTACAATGATAACGACAGCTTGGTACAGTGCACTTGGCATCCCTATAGATAGCTCGGGGAAGTTAGTTAATTACCCGCCAAATGGCAAATACCTTCTCAATCGTAAAAGGGACAACTTTGAGCAGACTTATAGCGATTTGATGAATAGGGTGGATTACATGACCAAAGTAAGGACTAAAATAGTCGGTGACGGAGACCGTAATTTCGGCTGCAGTCGCGGGTAATTTATTTTTTACAACAATGGCCTTTCTTATGGCTGGAAAGGCC  >  NC_000913/2760756‑2761216                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     ngcaaagcTTAAACATGATAAGCATATTAAAACTCAACGGAAAGACTGGCCTGATAGTCGACATTCCACTTTACGTTACGCATGGGTCAGAGAATATACCAAAAATAGAAAGCGGCATTACCATTTGATACTGTGTTTCAATCAGGATGCTTATTATCATTTAGGTGATTACGACTTAAACCGTAACACGTTACGTACAATGATAACGACAGCTTGATACAGTGCACTTGGCATCCCTATAGATAGCTCgg                                                                                                                                                                                                                    >  1:155742/2‑251 (MQ=255)                             aaaaCTCAACGGAAAGACTGGCCTGATAGTCGACATTCCACTTTACGTTACGCATGGGACAGCGACTATACCAAAAATAGAAAGCGGCATTACAATTTGATACTGTGTTTCAATCAGGATGTTTCTTATCATTTAGGTGATTACGACTTAAACCGTAACACGTTCCGTACAATGATAACGACAGCTTGATACAGTGCACTTGGCATCCCTATAGATAGCTCGGGGAAGTTAGTTAATTACCCGCCAAATgg                                                                                                                                                                                        >  1:231782/1‑251 (MQ=255)                                                                               cgCATGGGTCAGAGAATATACCAAAAATAGAAAGCGGCATTACCATTTGATACTGTGTTTCAATCAGGATGCTTATTATCATTTAGGTGATTACGACTTAAACCGTAACACGTTACGTACAATGATAACGACAGCTTGATACAGTGCACTTGGCATCCCTATAGATAGCTCGGGGAag                                                                                                                                                                                                               <  1:93345/178‑1 (MQ=255)                                                                                                                     aTTACCATTTGATACTGTGTTTCAATCAGGATGCTTATTATCATTTAGGTGATTACGACTTAAACCGTAACACGTTACGTACAATGATAACGACAGCTTGATACAGTGCACTTGGCATCCCTATAGATAGCTCGGGGAAGTTAGGTAATTACCCGCCAAATGGCAAATACCTTCGCAATCGTAAAAGGGACAACTTTGAGCAGACTTATAGCGATTTGATGAATAGGGTGGATTACATGACCAAAGTAAgg                                                                                                <  1:58500/251‑1 (MQ=255)                                                                                                                                  nnnnnnttnNNNNCAGNATGCTTATTATCATTTAGGTGATTACGACTTAAACCGTAACACGTTACGTACAATGATAACGACAGCTTGATACAGTGCACTTGGCATCCCTATAGATAGCTCGGGGAAGTTAGTTAATTACCCGCCAAATgg                                                                                                                                                                                        <  2:155742/144‑1 (MQ=255)                                                                                                                                     gtgtTTCAATCAGGATGCTTATTATCATTTAGGTGATTACGACTTAAACCGTAACACGTTACGTACAATGATAACGACAGCTTGATACAGTGCACTTGGCATCCCGATAGATAGCTCGGGGAATGTAGTGAATGACCCGCCAAATGGCATACACCTTCTCAATCGTAAAAGGGACAACTGTCAGCAGACTTAAAGGGATTTGATGAATAGGGTGGATTACATGACCAAAGTAAGGACTAAAATAGTCgg                                                                                  <  1:134859/249‑1 (MQ=255)                                                                                                                                                         attatCATTTAGGTGATTACGACTTAAATCGGAACACGTTACGTACAATGATAACGACAGCTTGATACAGTGCACTTGGCATCCCTATAGATAGCTCGGGGAAGTTAGTTAATTACCCGCCAAATGGCAAATACCTTCTCAATCGTAAAAGGGAc                                                                                                                                                            <  1:38874/155‑1 (MQ=255)                                                                                                                                                               aTTTAGGTGATTACGACTTAAACCGTAACACGTTACGTACAATGATAACGACAGCTTGATACAGTGCACTTGGCATCCCTATAGATAGCTCGGGGAAGTTAGTTAATTACCCGCCAAATGGCAAATACCTTCTCAATCGTAAAAGGGACAACTTTGAGCAGACTTATAGCGAtttn                                                                                                                                 <  1:29891/176‑2 (MQ=255)                                                                                                                                                                     gTGATTACGACTTAAACCGTAACACGTTACGTACAATGATAACGACAGCTTGATACAGTGCAAATGGCATCACTATAGATAGCTCGGGGAAGTTAGTTAATTACCCGCCAAATGGCAAATACCTTCTCAATCGTAAAAGGGAc                                                                                                                                                            >  1:178498/1‑143 (MQ=255)                                                                                                                                                                     gTGATTACGACTTAAACCGGAACACGTTACGTACAATGATAACGACAGCTTGATNCAGNGCACNGGNCATCCCTATAGATAGNTCGGAGAAGTTAGTTAATTACCCGCCAAATGGCAAATACCTTCTNNANNNTNAAAGGGAc                                                                                                                                                            <  2:178498/143‑1 (MQ=255)                                                                                                                                                                                       gTAACACGTTACGTACAATGATAACGACAGCTTCATACAGTGCACTTGGAATCCCTATAGATAGCTCGGGGAAGATAGTTAATTACCCGCAAAATGGCAAATACCTTCTCAATCGTAAAAGGGACAACTTTGAGCAGACTTATAGCGATTTGATGAATAGGGAGGATTACATGACCAAAGTAAGGACTAAAATAGTCGGTGACGGAGACCGTAATTTCGGCTGCAGTCGCGGGTACTTTATTTTTTacaac                              >  1:222695/1‑251 (MQ=255)                                                                                                                                                                                                                   aGCTTGATACAGTGCTCTTGGCATCCCTATAGATAGCTCGGGGAAGTTAGTTAATTACCCGCCAAATGGCAAATACCTTCTCAATCGTAAAAGGGACAACTTTGAGCAGACTTATAGCTATTTTATGAATAGGTTGGATTTCATGACCAAAGTATGGACTAAAATAGTCGGTGACGGAGAGCTTAATTTCGTCTGCAGTCGCTGGTAATTTATTTTTTACAACAATGGCCTTTCTTATGGCTGGAAAGGcc  <  1:34773/251‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     AGCAAAGCTTAAACATGATAAGCATATTAAAACTCAACGGAAAGACTGGCCTGATAGTCGACATTCCACTTTACGTTACGCATGGGTCAGAGAATATACCAAAAATAGAAAGCGGCATTACCATTTGATACTGTGTTTCAATCAGGATGCTTATTATCATTTAGGTGATTACGACTTAAACCGTAACACGTTACGTACAATGATAACGACAGCTTGGTACAGTGCACTTGGCATCCCTATAGATAGCTCGGGGAAGTTAGTTAATTACCCGCCAAATGGCAAATACCTTCTCAATCGTAAAAGGGACAACTTTGAGCAGACTTATAGCGATTTGATGAATAGGGTGGATTACATGACCAAAGTAAGGACTAAAATAGTCGGTGACGGAGACCGTAATTTCGGCTGCAGTCGCGGGTAATTTATTTTTTACAACAATGGCCTTTCTTATGGCTGGAAAGGCC  >  NC_000913/2760756‑2761216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑