Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	466,181	C→T	E378K (GAA→AAA)	ybaE ←	uncharacterized protein YbaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	466,181	0	C	T	88.9%	18.8 / NA	9	E378K (GAA→AAA)	ybaE	uncharacterized protein YbaE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (4/4);  minor base G (0/1);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCTCCGGTTTGCCGATGGTGATTTGCACGGGATGCCGACAACTGGTTCCCAAATCTTTTTCGAAAAGCGGCGGAGTTATCCAGTACTCAAC  >  NC_000913/465976‑466424                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    cAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGAATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGATTTCGGTAGTTTGAATTAATCCGGAACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGCACAGGGTTTTTTCGACTTCTAAAGTATGGAAAAAAACGGAATGAAGAATAATgg                                                                                                                                                                                                         >  1:87466/1‑250 (MQ=255)                                     naGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTTTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCgggg                                                                                                                                                                    >  1:151371/2‑251 (MQ=255)                                              gCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACATGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTTTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGACGGGGACTTTTgcg                                                                                                                                                           >  1:236527/1‑251 (MQ=255)                                                              gTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTTGCGGTGATCAGGTTTTGTCCGACTTCTAACGTTTGTAATAGACCGGATTTATGAATTATGTAGATCACGTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGc                                                                                                                                             <  1:139106/249‑1 (MQ=255)                                                                  gctttCTGCCATGGTTTTAAGTTCTAGTGGGAGGTGATAAACCAGCTTCAAGGTTGTCGGTAGTTTGACTTCATCCGGTCCCTGCCAATGCGGAATAGTCCAGCCTGTCAGTAATGCATGACCGGCGGTTATCAGGTTTTTTCCTACTTCTAACGTTTGTAATAAACCGGATTGAGGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGTACTTTGGCGCAACGTCAAATAGCAAAAAc                                                                                                                                       <  1:72626/248‑1 (MQ=255)                                                                                     aGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGC‑AATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTTTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAGTAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTg                                                                                                                   <  1:10966/251‑1 (MQ=255)                                                                                                                                                   ntGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGGTTTTTCCGACTTCTAACGTTTGTCATACACCAGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAgg                                                                                                                                                                               >  1:31465/2‑130 (MQ=255)                                                                                                                                                                          cTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTTTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAAAAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGATTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTTAACTGATGCCGccacgtacctg                                                                                                              <  1:62722/172‑11 (MQ=255)                                                                                                                                                                                                       aGGTTTTTTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTTACCCGTTGCAGCTCCTCCGGTTTGCCGATGGTGATTTGCACGGGATGCCGACAACTGGTTCCCAAATCTTTGTCGAAAAGCGGCGGAGTTATCCAGTACTCAAc  <  1:83090/251‑1 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    CAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCTCCGGTTTGCCGATGGTGATTTGCACGGGATGCCGACAACTGGTTCCCAAATCTTTTTCGAAAAGCGGCGGAGTTATCCAGTACTCAAC  >  NC_000913/465976‑466424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑