Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	715,506	C→T	A32V (GCT→GTT)	ybfP →	lipoprotein YbfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	715,506	0	C	T	100.0%	13.4 / NA	7	A32V (GCT→GTT)	ybfP	lipoprotein YbfP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/4);  total (3/4)

GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTT  >  NC_000913/715328‑715740                                                                                                                                                                                   |                                                                                                                                                                                                                                           gTAGGCCGGATAAGGCGTTCACGCAACATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGGCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGTTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATGGCAcn                                                                                                                                                                    <  1:49680/251‑2 (MQ=255)        ggATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGTTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCgg                                                                                                                                                             <  1:214160/251‑1 (MQ=255)                                                                                                                                               tgACGGCATGCCCCCACCCGGAACAATCATCATTGTTTGGCGACTGTTTACTACCCCCAAAAAATAAAACCAGAGAATTAAGGGGTATCATTGCGGTAAATGTTGCCCCTTGCGGGTGAAAGAAAAATTGCCGTGGTTATAATTTCCTTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGttn                       <  1:54870/250‑2 (MQ=255)                                                                                                                                                                  tGAACAATCATCATTAGTTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCAACTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATAACCGCCGCTGGCAGTTGTCAGGCggg                                                                                           >  1:143415/1‑163 (MQ=255)                                                                                                                                                                   gAACAATCATCATTAGTTGGCGACTGGTTACTGACCCCTAAAGATAAAACAAGAGGATTAACAGGTCGCCTTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGCTAACTTATCTGATAATACCCGCCGCTGGAAGTTGTCAGGAGGGAACGAAAAAGAACTTACTTATATTCATATTATGTCAGCACAAGAAAAGGTCGGTTTAAATACCGGTTGGCAATGCTACACCTCAttttt  >  1:192697/1‑251 (MQ=255)                                                                                                                                                                           atcatTAGTTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTAc                                                                                                                              <  1:19372/119‑1 (MQ=255)                                                                                                                                                                           atcatTAGTTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGNNTAAGAc                                                                                                                                                     >  2:19372/1‑96 (MQ=255)                                                                                                                                                                                   |                                                                                                                                                                                                                                           GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTT  >  NC_000913/715328‑715740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑