Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,830,658	C→T	A282A (GCC→GCT)	gutQ →	D‑arabinose 5‑phosphate isomerase GutQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,830,658	0	C	T	100.0%	13.2 / NA	8	A282A (GCC→GCT)	gutQ	D‑arabinose 5‑phosphate isomerase GutQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/2);  total (6/2)

GATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCTGGTGGCGGTATGTGAC‑GCTCAACAACAGGTACAAGGCGTCTTTACCGACGGCGATTTACGTCGCTGGCTGGTTGGCGGCGGCGCACTCACCACGCCAGTCAATGAAGCGATGACGGTCGGCGGCACCACGTTGCAATCGCAAAGTCGCGCCATCGACGCCAAAGAGATCCTGATGAAGCGCAAAATCACTGCCGCACCGGTGGTGGATGAAAACGGCAAACTCACCGGCGCAATAAACCTGCAGGATTTCTATCAGGCCGGGATTATTTAATCCTTCAATCCCAGACGTTTCGCCAGCCGATGCAGGTTGGCGACGTCGGTTTCCAGCATC  >  NC_000913/2830418‑2830838                                                                                                                                                                                                                                                  |                                                                                                                                                                                     gATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCTGGTGGCGGTATGTGAC‑GCTAAACAACAGGTACAAGGCGTCTTTACCGACGGCGATTTACGTCGCTGGCTGGTTGGCGGCGGCGCACTCACCACGCCAGTCAATGAAGCGATGACGGTCGGCGGCACCACGTTGCAATCGCAAAGTCGCGCTATCGACGccc                                                                                                                                                                            >  1:112815/1‑250 (MQ=255)                                                         cgatgcTGGAACTCAGCCGCACCGGTCTGGGGCTGGTGGCGGTATGTGAC‑GCTCCACAACAGGTACAAGGCGTCTTTACCGACGGCGATTTACGTCGCTGGCTGGTTGGCGGCGGCGAACTCACCACGCCAGTCAATGAAGCGATGACGGTCGGCGGCACCACGTTGCAATCGCAAAGTCGCGCTATCGACGCCAAAGAGATCCTGATGAAGCGCAAAATCACTGCCGCACCGGTGGTGGATGAAAACgac                                                                                                                    >  1:218407/1‑249 (MQ=255)                                                                        cccGCGCCGTTCTGGGGCTGTTGGCGGTATG‑GACCGCTCAACAACAGGTACAAGGCGTCTTTCCCGACGGCGATTTACGTCGCTGGCTGGTTGGCGGCGGCGCACGCACCCCGCCAGTCAATAAAGCGATGACGTTCGGCGGCACCACGTTGCAATCGCAAAGTCGCGCTATCGACGCCAAAAAGATCCTGATTAAGCTCAAAATCACGACCGAACCGGTGGTGGATGAAAACGGCAAACTCACCGgcgaa                                                                                                     <  1:236724/250‑3 (MQ=255)                                                                                               gCGGTATGTGAC‑GCTCAACAACAGGTACAAGGCGTCTTTACCGACGGCGACTTACGTCGCTGGCTGGTTGGCGGCGGCGCACTCACCAAGCCAGTAAATGAAGCGATGACGGTCGGCGGCACCACGTTGCCATCGCAAAGTCGCGCTATCGACGCCAAAGAGATCCTGATGAAGCGCAAAATCACTGCCGCACCGGTGGTGGATGACAACGGCAAACTCACCGGCGCAATAAACCTGCCGGATTTCTATc                                                                               >  1:180913/1‑250 (MQ=255)                                                                                                                                                     aCGTCGCTGGCTGGTTGGCGGCGGCGCACTCACCACGCCAGTCAATGAAGCGATGACGGTCGGCGGCACCACGTTGCAATCGCAAAGTCGCGCTATCGACGCCAAAGAGATCCTGATGAAGCTCAAAATCACTGCCGCACCGGTGGTGGATGAAAACGGCAAACTCACCGGCGCAATAAACCTGCAGGATTTCTATCAGGCCGGGATTATTTAATCCTTCAATCCCAGACGTTTCGCCAGCCGATGCAgg                          <  1:138972/250‑1 (MQ=255)                                                                                                                                                                  gTTGGCGGCGGCGCACTCACCACGCCCGTAAATGAAGCGATGACGGTCGGCGGAACCACGTTGCAATCGAAAAGTCGCGGTATGGACACCAAAGAGATCCTGACGAAGCGCAAAATCACCGACACACCGGTGGTGGATGAAAAAGGCATACTCACCGGCGCAATAAACCTGCAGGCTTTCTATCAGGCCGGGATTCTTTAATCATTCCATCCCAGACGTTTCGCCAGCAGATGCAGGTTGGCGAcggcggg            >  1:246017/1‑250 (MQ=255)                                                                                                                                                                            gcgcACTCACCACGCCAGTCAATGAAGCGATGACGGTCGGCGGCACCACGTTGCCATCGCAAAGTCGCGCTATCGACGCAAAAGAGATCCTGATGAAGCGCAAAATCACTGCCGCACCGGTGGTGGATGAAAACGGCAAACTCACCGGCGAAATAAACCTGCAGGATTTCTATCAGGCCGGGATTATTTAATCCTTCACTCCCAGACGTTTCGCCAGCCGATGCAGGTTAGCGACGTCGGTTTCCAGCATc  >  1:193518/1‑251 (MQ=255)                                                                                                                                                                            gcgcACTCACCACGCCAGTCAATGAAGCGATGACGGTCGGCGGCACCACGATGCAATCGAAAAGTCGCGATATCGACGCCACAGAGATCCTGATGAAGCGCAAAATCACTGCCGCACCGGTGGTGGATGAAAACGGCAAACTCACCGGCGCAATAAACCTGCAGGATTTCTATCAGGCCGGGATTATTTAATCCTTCAATCCCAGACGTTTCGCCAGCCGATGCAGGTTGGCGACg                 >  1:166657/1‑236 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                     GATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCTGGTGGCGGTATGTGAC‑GCTCAACAACAGGTACAAGGCGTCTTTACCGACGGCGATTTACGTCGCTGGCTGGTTGGCGGCGGCGCACTCACCACGCCAGTCAATGAAGCGATGACGGTCGGCGGCACCACGTTGCAATCGCAAAGTCGCGCCATCGACGCCAAAGAGATCCTGATGAAGCGCAAAATCACTGCCGCACCGGTGGTGGATGAAAACGGCAAACTCACCGGCGCAATAAACCTGCAGGATTTCTATCAGGCCGGGATTATTTAATCCTTCAATCCCAGACGTTTCGCCAGCCGATGCAGGTTGGCGACGTCGGTTTCCAGCATC  >  NC_000913/2830418‑2830838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑