Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,544,956	G→A	G218E (GGA→GAA)	fimC →	type 1 fimbriae periplasmic chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,544,956	0	G	A	100.0%	17.5 / NA	8	G218E (GGA→GAA)	fimC	type 1 fimbriae periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/6);  total (2/6)

GTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCATCGTTTGGCTGGTTTTT  >  NC_000913/4544815‑4545174                                                                                                                                              |                                                                                                                                                                                                                           gtAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGAAAGCAATATTACTTACCGAACAATAAATGAGTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTg                                                                                                               <  1:222072/251‑1 (MQ=255)                                           aCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGAAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTg                                                                                                                             <  1:137827/195‑1 (MQ=255)                                            ccTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGAAAGCAATATTACTTACCGAACACTAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAAtttt                                                                                                                                    >  1:208900/1‑187 (MQ=255)                                                   gTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGAAAGCAATATTACTTACCGAACAATAAATGATTATGGCGTACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAg                                                             <  1:127459/251‑1 (MQ=255)                                                                   ccGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGACGAAAGCATGGTTAAATTGCCTTCTGCTGCACACAGCAATATTACTTACAGAACATTAACTGCTTATGGCGCACTTACACCCCACATGAAGTGCGTAATGGAATAACCCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGACGGGt                                                                                                     >  1:238955/1‑195 (MQ=255)                                                                                       aaTGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGAAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTGTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCg                         <  1:138276/251‑1 (MQ=255)                                                                                                              cGAAAGCACGGTTAAATTGCCTTCTGATGCAGAAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCGCTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACGGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTGCCAGCGAAACACTCAATGCTTGCATATTCGTAAGCATCGTTTGGCTGGttttt  <  1:121975/251‑1 (MQ=255)                                                                                                                       ggTTAAATTGCCTTCTGATGCAGAAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACGTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTGTCGCCTGAAGAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCtn                                                                     <  1:25131/175‑2 (MQ=255)                                                                                                                                              |                                                                                                                                                                                                                           GTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCATCGTTTGGCTGGTTTTT  >  NC_000913/4544815‑4545174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑