Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,186	C→T	A127V (GCC→GTC)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,186	0	C	T	100.0%	29.4 / NA	12	A127V (GCC→GTC)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

TGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTC  >  NC_000913/45955‑46374                                                                                                                                                                                                                                        |                                                                                                                                                                                             tGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATAGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGGGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCACATCGTCACCTCAATGATCACCg                                                                                                                                                                             >  1:132960/1‑249 (MQ=255)             ggATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCAAATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAACTGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGTAGATTATCCCATCGTCACCTCAATGATCACCGAGTTCTCCAGTAcc                                                                                                                                                               >  1:235081/1‑251 (MQ=255)                                               gCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGTCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTAttn                                                                                                                                <  1:52790/248‑2 (MQ=255)                                                                                      tAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACAATGTTTGTTTCCTCCCCCGTCGAACTGTTGGAGCTGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATAGTCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGTCGCGACCTGTGCCGCTCTGGTCg                                                                                      >  1:238525/1‑251 (MQ=255)                                                                                                                                                   gCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTCTCGGCATTGTAATCGGTGACGATTATCCCATCGTCACCTCCATGATCACCGAGTTCTCCAGTCACCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGACGGCGGCTGGGGTTGGATGCTGGTTAGCgcggcg                              >  1:174696/1‑246 (MQ=255)                                                                                                                                                    cgacgaTGTTTGTTTCATCCCCCGTCGGACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGTCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCNATCNNNNNTann                                                                                                                                 >  2:149198/1‑144 (MQ=255)                                                                                                                                                    cgacgaTGTTTGTTTCATCCCCCGTCGACCTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGTCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTgn                                                      <  1:149198/221‑2 (MQ=255)                                                                                                                                                        gaTGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGTCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTc                                                                                                               <  1:136169/160‑1 (MQ=255)                                                                                                                                                                          cGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCTGTGCAGATTATCCCATCGTCACCTCAAGTATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTGTTCCATCAGCGTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGTCTACTGGCTTTATGATGTGGAAGGCGGTTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTc  <  1:181736/251‑1 (MQ=255)                                                                                                                                                                                                             gtGTCAGCGGTGCAGATTATCCCATCGTCACCTCAATGATCACCGAGTTCTCCAGTAc                                                                                                                                                                <  1:245217/57‑1 (MQ=255)                                                                                                                                                                                                                    cggggCAGATCATCCAAGCGTCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCNGCTNTATGNCCNCGATGTGTTATGTCGNCGCGACCTGTGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGNNGNNNGNTGGCGCTg                                                 <  2:175990/158‑1 (MQ=255)                                                                                                                                                                                                                    cGGTGCAGATTATCCCATCGTCACCTCAATGATCACCGAGCTCTCCAGTACAAGTCAGCGGGCGTTTTACCTCAGATTTATTGCCGCGCTGTGGTCTGCAGGCGCGCCCTGTGCCGATCTGGCCGGCCACTGGCCTTATGATGTGGAAGGCGGCTGGCGCTg                                                 >  1:175990/1‑162 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                             TGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTC  >  NC_000913/45955‑46374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑