Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	768,342	G→A	G122D (GGT→GAT)	mngB →	alpha‑mannosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	768,342	0	G	A	100.0%	19.5 / NA	9	G122D (GGT→GAT)	mngB	alpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/1);  total (8/1)

GAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGATGAAAATAGGTTATTTACCAGATTCCTTTGGCATGTCCGGGCAACTGCCGCATATCTACAATGGATTTGGCATTACCCGCACCATGTTCTGGCGCGGATGTTCGGAGCGCCACGGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGT  >  NC_000913/768101‑768565                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                gAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATAATCGAAGATTATTTCGCGCTGAAACCGGGAAACAAAGACCGTGTGACGAAACAGATAGAAGCCGGCAAGTTGATTCTCGGCCCCTGGTCTACCCAGACCGATACCACGATTGTTTCTGCGGAATACATCGTCCGTAATCTGATGTACGGACTGCGTGACTGCCTCGCGTTTGGCGAGCCGATGAAAATAGATTATTTAcc                                                                                                                                                                                                                        >  1:188455/1‑251 (MQ=255)                                                                      gTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGACTGCGTGACTGCCTCGCGTTTGGCGAGCCGATGAAAATAGATTATTTACCAGATTCCTTTGGCATGTCCGGGCAACTGCCGCATATCTACAATGGATTTGGCATTACCCGCACCATGtt                                                                                                                                                   >  1:233328/1‑251 (MQ=255)                                                                                                                                     cccTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGCATGCGTGACTGCCTCGCGTTTGGCGAGCCGATGAAAATAGATTATTTACCAGATTCCTTTGGCATGTCCGGGCAACTGCCGCATATCTACAATGGATTTGGCATTCCCCGCACCATGTTCTGGCGCGGCTGTTCGGAGCGCCACGGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTg                                                                                     >  1:121693/1‑250 (MQ=255)                                                                                                                                                           ncACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGATGAAAATAGATTATTTACCAGATTCCTTTGGCATGTCCGGGCAACTGCCGCATATCTACAATGGATTTGGCATTACCCGCACCATGTTCTGGCGCGGATGTTCGGAGCGCCACGGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGgcgc                                                                >  1:65283/2‑249 (MQ=255)                                                                                                                                                                                    cGTCCGTAATCTGAGTTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGATTAAAATAGATTATTTACCAGATTCCTTTGGCATGTCCGGGCAACTGCCGCATATCTACAATGGATTTGGCATTACCCGCACCATGTTCTGGCGCGGATGTTCGGAGCGCCACGGTACTTATAAAGCCGATTTTTTGGTGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATc                                      <  1:220641/250‑1 (MQ=255)                                                                                                                                                                                                   gTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGATGAAAATAGATTATTTACCAGATTCCTTTGGCATGTCCGGGc                                                                                                                                                                                                 >  1:165234/1‑80 (MQ=255)                                                                                                                                                                                                            gCGTGACTGACTCGCGTTTGGCGAGCCGATGAAAATAGATTATTTACCAGATTCCTTTGGCATGTCCGGGCAACTGCCGCATATCTACAATGGATTTGGCATTACCCGCACCATGTTCTGGCGCGGATGTTCGGAGCGCCACGGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGaa              >  1:206423/1‑250 (MQ=255)                                                                                                                                                                                                                        cgcgTTTGGCGAGCCGATGAAAATAGATTATTTACCAGATTACTTTGGCATGTCCGGGCAACTGCCGCATATCTACAATGGATTTGGCATTACCCGCACCATGTTCTGGCGCGGATGTTCGGAGCGCCACGGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGCTTACGt  >  1:103450/1‑250 (MQ=255)                                                                                                                                                                                                                                  gAGCCGCTGAAAATAGATTATTTACCAGATTCCTTTGGCATGTCCGGGCAACTGCCGCCTATCTACTATGGATTCGGCCTTACCCGCACCATGTTCt                                                                                                                                                 >  1:229204/1‑97 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                GAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGATGAAAATAGGTTATTTACCAGATTCCTTTGGCATGTCCGGGCAACTGCCGCATATCTACAATGGATTTGGCATTACCCGCACCATGTTCTGGCGCGGATGTTCGGAGCGCCACGGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGT  >  NC_000913/768101‑768565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑