Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	372,902	C→T	A263V (GCC→GTC)	mhpD →	2‑hydroxypentadienoate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	372,902	0	C	T	100.0%	24.2 / NA	11	A263V (GCC→GTC)	mhpD	2‑hydroxypentadienoate hydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/4);  total (7/4)

GATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATC  >  NC_000913/372666‑373133                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        gATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAATCCCAAAAGGAAGTc                                                                                                                                                                                                                           >  1:234154/1‑251 (MQ=255)                                                                               gctggcACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGTCCCAAAAGGAAg                                                                                                                                                                                                                             <  1:13900/171‑1 (MQ=255)                                                                                                                              atatCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGTCCCAAAAGGAAGTCTGTCATGNGTANNNNNa                                                                                                                                                                                                          >  2:153979/1‑143 (MQ=255)                                                                                                                              atatCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGTCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCgn                                                                                                                                                                                          <  1:153979/159‑2 (MQ=255)                                                                                                                                          ccGGGGCATTAGGGCCGATGGTGGCTGTGAATGCGGGAGATCGTTTTGAAGCCCATATTGAAGGCATAGGTGCAGGTGCTGCGACATTTTCAAGCGCAGTCTCAAAAGGAAGTCTGTCAGGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTCTGCTTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTgg                                                                                  <  1:228334/251‑1 (MQ=255)                                                                                                                                                      gTCCGATGGTGGCGATGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCCAGCGCCGTACCAAAAGGAATACTGTCATGAGTAAGCGTAAAGTCGCCCTTATCGGTTCTGGCAACATTTGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGGTGGCATTGATCCTCAGTCCGCCGGTCTAGCGCGCGCCAgg                                                                       >  1:191924/1‑249 (MQ=255)                                                                                                                                                             ggtggCGGTGAATGNGNGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGANATTTTCAAGCNCAGNNCCAANANGNATTCTGTCATGAGTAAGCGTAAAGTCGCCNTTATCGGTTCTGGCAACATTGGTNNCGNNNTGNNGATTa                                                                                                                                                          <  2:187007/160‑1 (MQ=255)                                                                                                                                                             ggtggCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGGGCAGTACCAAAAGGAAGTCTGTCATGGGTAAGCGGAAAGGCGCCCTTATAGGTTCTGGCCACATTGGTAACGATCTGATGAttt                                                                                                                                                          >  1:187007/1‑159 (MQ=255)                                                                                                                                                                                           aGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGTCCCAAAAGGACGTCTGTCATGAGTAAGCGTCAAGTCGCCATTATCGGTTCTGGCAACATTGGTCCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCgg                                 >  1:222383/1‑251 (MQ=255)                                                                                                                                                                                                                          gcGACATTTTCAAGCGCAGTCCCAAAAGGAAGCCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTCCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAgggg                                        >  1:214602/1‑213 (MQ=255)                                                                                                                                                                                                                          gcGACATTTGCAAGCGCAGTCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTAGCGTTTCTGGCAACATTGGTTCCGATCGGATGATTAAAATGATGCGTCACGGTCAGCAGCTGGAGATGGCGGTGATGGTTGGCATTGATCCGCAGTCCGGCGGTCGGGCGCGCGCCAGACGTATGGGCGTCGCCACCAGCCATGAAGTTGTGATCGTACTGATGAACATGCCTGAATTTGCTGATATc  <  1:167513/251‑1 (MQ=255)                                                                                                                                                                                                                           cGACATTTTCAAGCGCAGTCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATNNGATGATNna                                                                                                                                                         >  2:9411/1‑99 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        GATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATC  >  NC_000913/372666‑373133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑