Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,090,437	G→A	A31T (GCG→ACG)	endA →	DNA‑specific endonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,090,437	0	G	A	100.0%	21.0 / NA	9	A31T (GCG→ACG)	endA	DNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/5);  total (4/5)

CCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATTGCTGCGGTGGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTATCAATAGTTTTTCTCAGGCGAAAGCCGCGGCGGTAAAAGTCCACGCTGACGCGCCCGGTACGTTTTATTGCGGATGTAAAATTAACTGGCAGGGCAAAAAAGGCGTTGTTGATCTGCAATCGTGCGGCTATCAGGTGCGCAAAAATGAAAACCGCGCCAGCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATC  >  NC_000913/3090253‑3090674                                                                                                                                                                                         |                                                                                                                                                                                                                                              ccATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATTGCTGCGGTGGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTATCAATAGTTTTTCTCAGACGAAAg                                                                                                                                                                                                                                         >  1:57604/1‑191 (MQ=255)  cATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATTGCTGCGGTGGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTATCAATAGTTTTTCTCAGACGAAAGCCGCGGCGGTAAAAGTCCACGCTGACGCGCCCGGTACGTTTTATTGCGGATGTAAAATTa                                                                                                                                                                             >  1:6229/1‑250 (MQ=255)                                     aTTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACAGTTATTTGTCTATTGCTGCGGTGGAACTGAGCGCAGCATTTTCCGGCCCGGAGTTGGCCGAAGGTATAAATAGTTTTTCTAAGACGAAAGCCGCGGCGGTAAAAGACCGCGATGACGCGCCCGGTCCGGTTTTTTGCGGATGTaaa                                                                                                                                                                                 >  1:116143/1‑211 (MQ=255)                                                                          cGTTTTAACANGGAGTAAGTGACGTACCGTTATTTGTCTATTNCNGCGGTGGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTNTCAATAGTCTNTCTNNGACTNANGNCGCTGCGGTAAAAGTCCACGCTGACGCGCNCGGTACGTTTTAGTGCGGATGTANNTTNNNCTNNCAggg                                                                                                                                                                   <  2:191624/188‑1 (MQ=255)                                                                          cGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTTTTGCTGCGGTGGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGGATCAATATTTTTTCTCAGACGAAAGCCGCGGCGGGAAAAGTCCACGCTTACGCGCCCGGTACGTTTTATTGCGGATGTAAAATTAACTGGCAGGGCAAAAAATGCGTTGTTGATCTGCAATCGTGCGTCTATCAGGGGCTCAAAAATGAAAACCgcgc                                                                                                    <  1:222468/251‑1 (MQ=255)                                                                          cGTTTTAACACGGAGCAAGTGATGTACCGTTATTTGTCTATTGCTGCGGTAGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTATCAATAGTTTTTCTCAGACGCAAGCCGCGGCGGTAAAAGTCCACGCTGAAGCGCCCGGTACGTTTTATTGCGGATGTAAAATTAACTGGCAggg                                                                                                                                                                   >  1:191624/1‑188 (MQ=255)                                                                                                                                              ntCNGGCCCNGCGTNGGCCGAAGGTATCAANAGTTTTTCGCAGACTAAACNNGNGGCGTNAAAAg                                                                                                                                                                                                                          <  2:112095/64‑1 (MQ=255)                                                                                                                                                               ccGAAGGTATCAATAGGTTGTCTCAGACGAAAGCCGCGGCGGTAAAAGTCCACGCTGACGCGCCCGGTACGTTTTAGTGCGGATGTAAAATTAACTGGCAGTGCAAAAAAGGCGTTTTTGATCTGCAATCGTGCGGCGATCAGGTGCGCAAAAAGTAAAACCGCGCCAGCCGCGTAGAGTGGGAACATGTCGTTc                                                                       <  1:188872/195‑1 (MQ=255)                                                                                                                                                                             aGTTTTTCTCAGACGAAAGCCGCGGCGGTAAAAGTCCACGCTGACGCGCCCGGTACGTTTTATGGCGGATGTAAAATTAACTGGCAGGGCAAAAAAGGCGTTGTTTATCTGCATTCGTGCGGCTATCATGTGCGCGAAAATGAAAACCGCGGCAGCCGCTGAGAGTGGGAACATGTCGTTCCCTCGTGGCATGTCGTGCAGCAGCGCCGGTTCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGacc  <  1:232903/250‑3 (MQ=255)                                                                                                                                                                                         |                                                                                                                                                                                                                                              CCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATTGCTGCGGTGGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTATCAATAGTTTTTCTCAGGCGAAAGCCGCGGCGGTAAAAGTCCACGCTGACGCGCCCGGTACGTTTTATTGCGGATGTAAAATTAACTGGCAGGGCAAAAAAGGCGTTGTTGATCTGCAATCGTGCGGCTATCAGGTGCGCAAAAATGAAAACCGCGCCAGCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATC  >  NC_000913/3090253‑3090674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑